豌豆病程相关蛋白应答GSH的蛋白质组学分析  

A Proteomic Approach to Study Defense-Related Proteins Responses to GSH Treatment in Pea (Pisum sativum)

作　　者：刘红霞[1] 赵鑫[1,2] 毕阳[2] 张增艳[1] 陈佰鸿[2] 安成才[3] 

机构地区：[1]中国农业科学院作物科学研究所/农作物基因资源与基因改良国家重大科学工程/农业部作物遗传育种重点开放实验室,北京100081 [2]甘肃农业大学农学院,兰州730070 [3]北京大学生命科学学院,北京100871

出　　处：《中国农业科学》2010年第22期4746-4753,共8页Scientia Agricultura Sinica

基　　金：国家自然科学基金项目(30500342);中国博士后项目(2004036176)

摘　　要：【目的】为揭示豌豆的抗病反应机理,采用蛋白质组学方法对豌豆上胚轴组织中应答GSH的抗病防御蛋白质种类和信息进行分析研究。【方法】以50mmol·L-1还原性谷胱苷肽(reduced glutathione,GSH)作为抗病激发子,利用高通量2-DE和MALDI-TOFMS质谱技术大规模分离和鉴定豌豆白化苗中与抗病防御相关的差异蛋白质点。【结果】从GSH处理48h的豌豆白化苗上胚轴组织中共分离筛选得到4种蛋白质与豌豆的抗病防御反应密切相关,包括病程相关蛋白PR10(PI49)、脱落酸胁迫响应蛋白ABR17和ABR18及1个富含甘氨酸的RNA束缚蛋白(GRPs)。发现ABR17与PI49在GSH逆境胁迫下相伴出现,二者交互作用可能提高豌豆的抗病性。【结论】在GSH侵染条件下,豌豆上胚轴在48h内大量积累PR和ABA胁迫响应等大分子量抗菌蛋白,可能依靠这些病程相关蛋白的积累,增强豌豆对侵染真菌的抗病能力。【Objective】 This study was conducted to isolate and identify the defense-related proteins in pea albino seedlings in response to GSH treatment, and to clarify the disease-resistant mechanism of these proteins involved in pea disease response. 【Method】 The method of proteomic analysis was used in the study. Four kinds of defense-related proteins closely related to pea disease response were successfully isolated from pea albino seedlings treated with 50 mmol·L-1 reduced glutathione （GSH） after 48 h of treatment. After the identification with MALDI-TOF MS, a pathogenesis-related protein PR10 （PI49）, two ABA responsive proteins ABR17 and ABR18, and a glycine-rich RNA binding protein （GRPs） were identified. The possible roles of these four proteins in the disease response of pea seedlings to GSH treatment were discussed. 【Result】 Results showed that ABR17 and PI49 emerged concomitantly, indicating a potential cooperative effects of these two proteins in pea disease response to enhanced disease-resistance of pea. 【Conclusion】 Under GSH treatment, PR and ABA responsive proteins were the main anti-fungal proteins at 48 h after treatment, indicating that these two proteins would play pivotal roles in pea disease response to pathogen attacks.

关 键 词：豌豆 病程相关蛋白 2-DE 飞行质谱 GSH 

分 类 号：S436.43[农业科学—农业昆虫与害虫防治]