毛细管电泳-间接紫外法检测蜂蜜中的多种金属离子  

Determination of Metal Ions in Honey by Capillary Electrophoresis with Indirect UV Method

作　　者：石美[1] 冯加民[1] 陆永超[1] 高庆宇[1] 

机构地区：[1]中国矿业大学化工学院,江苏徐州221116

出　　处：《分析测试学报》2010年第11期1159-1164,共6页Journal of Instrumental Analysis

基　　金：国家自然科学基金资助项目(20943005);江苏省科学基金资助项目(BK2007037);中国矿业大学青年基金资助项目(OH060104)

摘　　要：探讨了一种利用毛细管电泳测定K+、Ba2+、Ca2+、Na+、Mg2+、Fe2+、Zn2+、Cd2+、Li+和Cu2+10种金属离子的方法。考察了紫外生色剂的浓度和pH值对分离效果的影响,并优化了分离条件。结果表明,咪唑浓度为15 mmol/L(pH 3.7),分离电压为20kV时,10种金属离子可在7min内实现基线分离。10种金属离子的线性范围为0.06～60.0mg/L,相关系数为0.9995～0.9999,检出限为0.01～0.21mg/L。标准样品的回收率为95%～104%,5次测定的相对标准偏差为0.9%～5.8%。将该法用于6个蜂蜜样品的测定,结果满意。A capillary electrophoresis coupled with indirect UV method was developed to separate ten metal ions, including K^＋, Ba^2＋, Ca^2＋, Na^＋, Mg^2＋, Fe^2＋, Zn^2＋, Cd^2＋, Li^＋ and Cu^2＋. The .effects of chromophore imidazole concentration and pH value on separation efficiency were investigated. The results indicated that all the cations could be completely separated within 7 rain by using 15 mmol·L^-1 imidazole （ pH = 3.7 ） as background electrolyte and 20 kV as a running voltage at 25 ℃ The calibration curves for ten metal ions were linear in the range of 0.06 - 60.0 mg/L with relative coefficients of 0. 999 5 - 0. 999 9. The limits of detection were in the range of 0. 01 - 0. 21 mg/L. The spiked recoveries were in the range of 95% - 104% with RSDs（n = 6） of 0. 9% - 5.8% . The method was applied in the determination of ten metal ions in six honey samples with satisfactory resuits.

关 键 词：毛细管电泳 间接紫外法 蜂蜜 金属离子 

分 类 号：O657.8[理学—分析化学] S896.1[理学—化学]