联合检测ΔNp63、Ki-67和VEGF与膀胱移行细胞癌预后的相关性研究  

Study on ralationship of combined detection of expressions of ΔNp63,Ki-67,VEGF and prognosis of bladder cancer

作　　者：伍季[1] 苟欣[2] 李云祥[1] 

机构地区：[1]川北医学院第二临床学院泌尿外科,四川南充637000 [2]重庆医科大学附属第一医院泌尿外科,400162

出　　处：《重庆医学》2010年第22期3056-3058,共3页Chongqing medicine

摘　　要：目的探讨ΔNp63、Ki-67和血管内皮生长因子(VEGF)在膀胱移行细胞癌(TCCB)中的免疫组化表达及与膀胱癌病理分级、临床病理分期和预后的相关性。方法用SP法对56例膀胱移行上皮癌病理切片行ΔNp63、Ki-67和VEGF免疫组化染色,将免疫组化染色结果与病理分级、分期和预后进行分析。结果 TCCB中ΔNp63、Ki-67和VEGF阳性表达率明显高于正常膀胱黏膜,在高级别、浸润性癌组织中的阳性表达率明显高于低级别、浅表性癌组织,在复发患者中的阳性表达率显著高于初发组,在膀胱癌的病理分级、临床分期和预后之间表达差异有统计学意义(P<0.05)。采用Spearman等级相关性分析,ΔNp63与Ki-67、VEGF呈正相关,差异均有统计学意义(P<0.05)。结论ΔNp63可能通过促进细胞增殖和肿瘤血管生成发挥促癌作用,联合检测ΔNp63、Ki-67和VEGF多项肿瘤标记物可以更好地判断TCCB的预后。Objective To evaluate the immunohistochemistry expressions of ΔNp63,Ki-67,VEGF in transitional cell carcinoma of bladder and the relationship between the expressions of ΔNp63,Ki-67,VEGF and the pathological stage,grade and prognosis of bladder cancer.Methods To detect the expressions of ΔNp63,Ki-67 and VEGF on these pathological sections by immunohistochemical staining.The immunoassay results were analyzed in relation to the tumor stage,grade and compared them with the clinical parameters of prognosis.Results The expressions of TCC-positive rate of ΔNp63,Ki-67 and VEGF were higher than those of the cell of normal bladder mucous membrane;the expressions of positive rate of ΔNp63,Ki-67 and VEGF in the high-grade and the invasive TCCB were significantly higher than those of low-grade and the superficial TCCB.The expressions of TCC-positive rate of ΔNp63,Ki-67 and VEGF in the recurred cases were much higher than those of the primary cases.The expression difference of the three marker in the pathology grade,clinical stage and prognosis had statistical significance（P〈0.05）.To compare and analyze three markers expression in the bladder cancer by using Spearman rank correlation,ΔNp63 and Ki-67,ΔNp63 and VEGF showed positive correlation,rank correlation had statistical significance（P〈0.05）.Conclusion ΔNp63 may play a role of promoter in create cancer′s genesis through the promotion of cell proliferation and blood vessel form.The combined detection of the expressions of ΔNp63,Ki-67 and VEGF can give a better judgment on prognosis of bladder cancer.

关 键 词：膀胱癌 免疫组化 ΔNP63 KI-67 血管内皮生长因子 

分 类 号：R737.14[医药卫生—肿瘤] R730.45[医药卫生—临床医学]