大孔疏水载体的制备及其在脂肪酶固定化中的应用  

Preparation of a macroporous hydrophobic poly(styrene-divinylbenzene) support and its application in lipase immobilization

作　　者：付大雁[1] 蔡宏举[1] 周鑫[1] 秦培勇[1] 谭天伟[1] 

机构地区：[1]北京化工大学生命科学与技术学院,北京100029

出　　处：《北京化工大学学报（自然科学版）》2010年第6期116-120,共5页Journal of Beijing University of Chemical Technology(Natural Science Edition)

基　　金：国家自然科学基金(20636010);国家"863"计划(2007AA100404);国家"973"计划(2007CB714304)

摘　　要：以苯乙烯(St)为功能单体,二乙烯基苯(DVB)为交联剂,采用固液联合致孔的方式,制备了疏水多孔载体。将多孔载体用于脂肪酶Candida sp.99-125的固定化,得780U/g的固定化活力。固定化使酶在30～50℃范围内热稳定性得到了明显的提高,最适反应温度提高了5℃,在pH6.0～9.0范围内酶的相对活力有所提高,最适pH不变仍为8.0。固定化酶单次催化油酸和十六醇的酯化率达98%,其操作稳定性好,连续间歇操作15批,酯化率仍可达到50%。A macroporous hydrophobic poly（styrene-divinylbenzene） support has been prepared using a multiple porogen and used for the immobilization of Candida sp. 99-125 lipase. The conditions of the lipase immobilization on the support were optimized and an optimum activity （780 U/g） was obtained. After immobilization,the thermal stability of the lipase was increased in the temperature range 30-50 ℃,and its stability in the pH range 6.0-9.0 was also increased. The optimum temperature after immobilization was increased by 5 ℃. The enzyme preparation had increased relative activity at pH 6.0-9.0 and a maximum activity at pH 8.0. The esterification reaction of cetyl alcohol and oleic acid was efficiently catalyzed by the enzyme preparation with the yield of ester being 98%. Operational stability tests indicated that the ester yield was still as high as 50% after 15 batch cycles.

关 键 词：大孔载体 脂肪酶 固定化 

分 类 号：Q814.2[生物学—生物工程]