胃癌VEGF-C、VEGFR-3及CNTN-1的表达及其与淋巴转移的关系  

Expressions of VEGF-C,VEGFR-3 and CNTN-1 and their significance on lymphatic metastasis in gastric cancer

作　　者：吴生华[1] 俞继卫[1] 郑林海[1] 吴巨钢[1] 倪晓春[1] 李晓强[2] 杜光烨[2] 姜波健[1] 

机构地区：[1]上海交通大学医学院附属第三人民医院普通外科,上海201900 [2]上海交通大学医学院附属第三人民医院病理科,上海201900

出　　处：《中国普通外科杂志》2010年第10期1065-1070,共6页China Journal of General Surgery

基　　金：上海交通大学医学院科研基金赞助(2007XJ035;2009XJ21037);上海市科委基金赞助(09411962300)

摘　　要：目的探讨血管内皮生长因子-C(VEGF-C)及其受体VEGFR-3(也称Flt-4)与神经细胞黏附分子-接触蛋白-1(contactin-1,CNTN-1)在原发性胃癌组织中的表达,并分析其与淋巴转移和其他临床病理参数之间的关系。方法对37例原发性胃癌病例的原发灶和癌旁组织标本,行免疫组化法检测VEGF-C、VEGFR-3及CNTN-1的表达,测定淋巴管密度(lymphatic vessel density,LVD)。分析上述四者之间的相互关系,及其与临床病理因素之间的联系。结果胃癌组织中VEGF-C、VEGFR-3及CNTN-1的阳性表达率分别为54.05%、72.97%及62.16%。CNTN-1的表达与VEGF-C(P<0.05)及VEGFR-3表达(P<0.05)存在明显的相关性。VEGF-C的阳性表达与TNM分期、淋巴管浸润及淋巴结转移有关(均P<0.05)。肿瘤较大、TNM分期、淋巴管浸润和淋巴结转移率较高者,CNTN-1表达的阳性率也越高(均P<0.05)。VEGFR-3阳性表达与TNM分期及有淋巴结转移有关(均P<0.05)。LVD计数与TNM分期晚、淋巴管浸润、淋巴结转移有关(P<0.05)。LVD计数与VEGF-C的表达呈正相关(P<0.05)。结论 VEGF-C、VEGFR-3和CNTN-1在胃癌组织中均存在高表达。VEGF-C的表达与LVD计数相关,且两者都与淋巴管浸润和淋巴结转移密切相关。VEGF-C可能是通过CNTN-1通路介导淋巴管生成,从而促进胃癌的淋巴管浸润和淋巴结转移。Objective To determine the relationship between vascular endothelial growth factor-C （VEGF-C）,vascular endothelial growth factor receptor-3 （VEGFR-3,also known as Flt-4） and CNTN-1 （contactin-1） expression in human gastric cancer,as well as the correlation of them with metastatic lymph nodes and other clinicopathologic parameters.Methods The samples from primary foci of 37 cases with primary gastric cancer were immunohistochemically examined for VEGF-C,VEGFR-3 and CNTN-1 expressions.Assessment of lymphatic vessel density （LVD） was undertaken.Then we analyzed their relationships and correlations with clinicopathologic findings.Results The positivity rate of VEGF-C,VEGFR-3 and CNTN-1 in the primary tumor was 54.05%,72.97% and 64.16% respectively.The expression of CNTN-1 significantly correlated with VEGF-C （P0.05） and VEGFR-3 （P0.05）.VEFG-C was correlated to TNM stage,lymphatic vessel invasion and lymph node involvement （P0.05）.CNTN-1 expression was associated with tumor size,TNM stage,lymphatic vessel invasion and lymph node metastasis （P0.05）.Higher expression of VEGFR-3 was detected in the patients with advanced TNM stage and lymphatic node metastasis （P0.05）.Increased level of LVD was significantly associated with later TNM stage,lymphatic vessel invasion and lymph node metastasis （P0.05）.LVD was positively correlated with VEGF-C expression （P0.05）.Conclusions High expression of VEGF-C,VEGFR-3 and CNTN-1 was observed in gastric cancer.The expression of VEGF-C correlates with LVD expression and both of them correlate with the presence of lymphatic vessel invasion and lymph node metastasis.VEGF-C-mediated CNTN-1 overexpression might promote lymphatic vessel invasion and lymph node metastasis via lymphangiogenesis pathway in patients with gastric cancer.

关 键 词：胃 新生物/癌 接触蛋白-1 血管内皮生长因子 淋巴转移 

分 类 号：R73-37[医药卫生—肿瘤]