肝细胞生长因子和血管内皮生长因子C在非小细胞肺癌中的共表达及与淋巴管生成的相关性  

Co-expression of hepatocyte growth factor and vascular endothelial growth factor-c in non-small cell lung cancer and their relationship with lymphangiogenesis

作　　者：林文俐[1] 张楠[2] 曲飞[3] 刘杰[2] 王婧男[4] 解田燕[3] 孙玉萍[2] 

机构地区：[1]山东大学医学院,济南250012 [2]山东大学附属济南市中心医院肿瘤科,济南250013 [3]山东大学附属济南市中心医院病理科,济南250013 [4]山东省医学科学院基础医学研究所,济南250062

出　　处：《山东大学学报（医学版）》2010年第10期111-115,共5页Journal of Shandong University:Health Sciences

基　　金：济南市科技明星计划资助课题(济青科合字2007第1-115号)

摘　　要：目的检测肝细胞生长因子(HGF)和血管内皮生长因子C(VEGF-C)在非小细胞肺癌(NSCLC)组织中的表达,分析两者的相关性及其与NSCLC淋巴管生成的关系。方法应用免疫组织化学SP法,检测72例NSCLC患者及20例癌旁正常肺组织中HGF和VEGF-C的表达,用D2-40标记淋巴管,计数微淋巴管密度(LMVD),分析HGF和VEGF-C共表达情况及与LMVD和临床病理参数之间的关系。结果 HGF和VEGF-C在NSCLC中癌组织的表达明显高于癌旁正常肺组织(31.94%vs15.00%、48.65%vs25.00%,P均<0.05),且两者存在明显正相关(r=0.170,P<0.05);HGF或VEGF-C阳性表达的LMVD显著增高[HGF:(16.98±4.31)vs(13.06±4.28),P<0.05;VEGF-C:(19.43±3.24)vs(14.66±3.51),P<0.05],且与患者淋巴结转移成正相关(P均<0.05)。结论 HGF和VEGF-C在NSCLC中存在共表达,且两者均与肿瘤淋巴管的生成及淋巴道的转移密切相关。Objective To determine expressions of hepatocyte growth factor（HGF） and vascular endothelial growth factor-c（VEGF-C） and their relevance,and evaluate the relationship with lymphangiogenesis in non-small cell lung cancer（NSCLC）.Methods Applying the immunohistochemical SP method,lymphatic vessels and expressions of HGF and VEGF-C were detected in 72 non-small cell lung cancer tissues and 20 adjacent normal tissues.Lymphatic micro-vessel density（LMVD） was counted with D2-40 as the specific marker of the lymphatic endothelium.The relationship of HGF and VEGF-C with LMVD and the clinicopathological features of NSCLC were then analyzed.Results HGF/VEGF-C positive expression rates were significantly higher than those in adjacent normal tissues（31.94% vs 15.00%,48.65% vs 25.00%,both P0.05）,and there was a positive correlation between expressions of HGF and VEGF-C（r=0.170,P0.05）.Higher LMVD was found in NSCLC with HGF or VEGF-C positive expression [HGF：（16.98±4.31） vs（13.06±4.28）,P0.05;VEGF-C：（19.43±3.24） vs（14.66±3.51）,P0.05].Expressionsof HGF and VEGF-C were positively correlated with lymph node metastasis（both P0.05）.Conclusion In non-small cell lung cancer,co-expression of HGF and VEGF-C can promote lymphangiogenesis and lymphatic metastasis.

关 键 词：癌 非小细胞肺 肝细胞生长因子 血管内皮生长因子C 淋巴管生成 

分 类 号：R734.2[医药卫生—肿瘤]