ZD6474对鼻咽癌细胞株CNE2抑制作用及其机制的探讨  

Inhibitory effect of ZD6474 on nasopharyngeal carcinoma cell CNE2 and its mechanism

作　　者：陈卫国[1,2] 袁亚维[1] 

机构地区：[1]南方医科大学南方医院放疗科,广东广州510515 [2]萍乡市人民医院消化科,江西萍乡337000

出　　处：《中华肿瘤防治杂志》2010年第19期1514-1516,共3页Chinese Journal of Cancer Prevention and Treatment

摘　　要：目的:研究ZD6474对鼻咽癌细胞株CNE2的抑制作用。方法:采用MTT法测定ZD6474的抑制浓度(IC50),流式细胞仪检测ZD6474的凋亡率及细胞周期分布。结果:MTT数据显示,CNE2细胞的抑制率与ZD6474浓度成正相关,随ZD6474浓度升高,抑制作用越明显,IC50=2.531μmol/L;流式细胞术分析显示,ZD6474处理48h使CNE2细胞凋亡率明显升高(24.02±5.90)%,且随浓度升高凋亡率升高越明显,P<0.001。细胞周期结果显示,ZD6474使细胞周期S期(34.96±5.37)%比例下降,G0/G1期(56.25±1.45)%和G2/M期(17.36±5.73)%比例升高。结论:ZD6474能明显抑制CNE2细胞的增殖,促进其凋亡并改变其细胞周期的分布。OBJECTIVE：To investigate the effect of ZD6474,a tyrosine kinase inhibitor on nasopharyngeal carcinoma CNE2 cell line. METHODS： The antiproliferative and proapoptotie activity of ZD6474 in CNE2 ceil line were detected with MTT method and flow cytometry. RESULTS： With diferent concentrations of ZD6474,a dose-dependent growth inhibition was observed in CNE2 cell line and IC50 was 2.531 μmol/L.Flow cytometry analysis showed that,ZD6474 treatment 48 h to CNE2 cell apoptosis rate increased significantly （24.02±5.90）%,and the apoptosis rate increased with concentration more significantly （P0.001）. The results showed that ZD6474 could edcrease the percontage of S phase （34.96±5.37）%,and inereased the percentage of G0/G1 phase （56.25±1.45）% and G2/M phase （17.36±5.73）%. CONCLUSION： This study shows ZD6474 has remarkably anti proliferative and proapoptotie effect on nasopharyngeal carcinoma ceil line CNE2 in vitro.

关 键 词：鼻咽肿瘤 细胞系 细胞周期 细胞凋亡 受体蛋白质酪氨酸激梅类 流式细胞术 

分 类 号：R739.63[医药卫生—肿瘤]