汉防己碱对MCF-7细胞凋亡及Akt/NF-κB信号传导通路的影响  

Influence of tetrandrine on apoptosis and AKT/NF-κB signal pathway in human MCF-7 cell line

作　　者：武建毅[1] 沈清[1] 金赟洁[1] 姚晓祥[1] 朱纪华[1] 朱麟玉[1] 黄晓东[1] 

机构地区：[1]上海市普陀区人民医院肿瘤科,上海200060

出　　处：《中华肿瘤防治杂志》2010年第19期1524-1527,共4页Chinese Journal of Cancer Prevention and Treatment

基　　金：江苏大学医学科技发展基金(JLY20080095)

摘　　要：目的:探讨汉防己碱诱导乳腺癌细胞MCF-7凋亡的信号机制。方法:以MTT法测定不同浓度的汉防己碱(1、5和10μmol/L)对MCF-7肿瘤细胞增殖抑制;流式细胞仪检测细胞凋亡;免疫印迹法分析AKT活化;细胞免疫化学染色检测NF-κB表达。结果:不同浓度汉防己碱对培养的乳腺癌细胞MCF-7生长有显著的抑制作用,有效的诱导凋亡,且呈剂量依赖性,各组MCF-7细胞生长抑制率分别为(34.04±2.06)%、(54.07±1.08)%和(70.29±1.05)%,与对照相比较差异均有统计学意义,P<0.01;各组细胞凋亡率分别为(15.21±2.13)%、(23.07±1.08)%和(30.35±1.06)%,与对照相比差异均有统计学意义,P<0.05。汉防己碱对细胞内总AKT激酶蛋白的表达没有影响,但抑制磷酸化AKT蛋白的表达;同时对细胞内NF-κB的表达有抑制作用。结论:汉防己碱诱导乳腺癌细胞凋亡的机制与Akt/NF-κB信号途径有关。OBJECTIVE：To study the cell signal mechanism of tetrandrine on apoptosis of human breast cancer cell line MCF-7. METHODS： The effects of different doses （1, 5 and 10 μmol/L） of tetrandrine on the proliferation of MCF-7cells were observed by MTT assay. Apoptosis was detected with flow cytometry. Western blot was performed to assess ser473-phosphorylaed Akt. Immuno-cytochemistry was used to analyze the expression of NF-κB. RESULTS： The different doses of tetrandrine inhibited the proliferation and induced the apoptosis of MCF-7 in a dose-dependent manner, and the inhibitory rates of proliferation in MCF-7 cells were （34.04±2.06）%,（54.07±1.08）% and （70.29±1.05）% respectively （P0.01）; the rates of apoptosis were （15.21±2.13）%, （23.07%±1.08）% and （30.35±1.06）% respectively （P0.05）. Tetrandrine could significantly inhibite serine 473 phosphorylation in Akt and the expression of NF-κB. CONCLUSION： Tetrandrine can induce apoptosis in MCF-7 cells through the Akt/NF-κB signal pathway.

关 键 词：汉防己碱 乳腺肿瘤 细胞凋亡 NF-ΚB 信号传导 

分 类 号：R737.9[医药卫生—肿瘤]