福建肝癌细胞EBV感染与HBV及P53蛋白表达的关系  

Relationship between EBV infection in Fujian HCC and HBV and P53 protein expression *

作　　者：袁芳平[1] 黄培生[1] 王镛[1] 龚华实[1] 

机构地区：[1]福建医科大学病理解剖学教研室

出　　处：《世界华人消化杂志》1999年第6期491-493,共3页World Chinese Journal of Digestology

基　　金：福建省科委八五重点攻关项目

摘　　要：目的探讨肝细胞癌（ＨＣＣ）中ＥＢＶ与ＨＢＶ感染的相关性及ＥＢＶ与Ｐ５３蛋白表达的关系．方法采用原位杂交技术以地高辛标记的单链ｃＤＮＡ探针、生物素标记的双链ｃＤＮＡ探针及免疫组化ＳＰ法检测ＨＣＣ５９例（含癌旁肝组织）和９例肝硬变组织中高拷贝数ＥＢＥＲ１（ＥＢＶ编码的小ＲＮＡ）、ＨＢＶＤＮＡ和Ｐ５３蛋白．结果肝癌细胞核内ＥＢＥＲ１阳性率为２０３％，显著高于癌旁肝组织（Ｐ＜００１）．肝癌组织内ＨＢＶＤＮＡ阳性率为５９３％，ＥＢＶ存在与ＨＢＶ感染无明显相关性（Ｐ＞００５）．肝癌组织内Ｐ５３蛋白表达率为３３９％，Ｐ５３表达与ＥＢＥＲ１无显著关系（Ｐ＞００５）．结论肝癌细胞内ＥＢＶ感染与ＨＢＶ存在无明显关系。AIM To study the correlation of EBV and HBV infection in the hepatocellular carcinoma(HCC), and the relationship between EBER 1 and P53 protein in HCC. METHODS EBER 1(EBV encoded small RNA),HBVDNA and P53 protein of 59 cases of HCC including surrounding liver tissues and 9 cases of liver cirrhosis(LC) were detected using in situ hybridization with digoxin labeling single strand cDNA probe, biotin labeling double strand cDNA probe and streptavidin peroxidase(SP) immunohistochemistry respectively. RESULTS BEER 1 expression was found in the nuclei of tumor cells in 12/59 (20 3%) cases of HCC. No expression was seen in paratumorous liver tissues including dysplasia liver cells, infiltrating lymphocytes and liver cirrhosis cases. The positive rate of HBVDNA was 59 3% in HCC tissues. There was no relationship between EBER 1 and HBVDNA ( P >0 05). P53 protein expression was shown in 20/59 (33 9%). No expression was found in the surrounding nontumors liver tissues. P53 protein expression was independent of EBER 1 ( P >0 05). CONCLUSION EBV infection is independent of HBV in HCC tissues. There is no relationship between the P53 expression and EBER 1 in HCC.

关 键 词：肝肿瘤 病理学 疱疹病毒 HBV P53蛋白 

分 类 号：R735.702[医药卫生—肿瘤]