外源性表皮生长因子治疗大鼠慢性胃溃疡对原癌基因表达的影响  

Effect of exogenous EGF on proto oncogene expression in experimental gastric ulcer in rats

作　　者：陈宝雯[1,2] 王洪涛[1,2] 刘正新[1,2] 贾博琦[1,2] 马清钧 

机构地区：[1]北京医科大学第一医院消化科 [2]军事医学科学院生物工程研究所分子遗传室

出　　处：《世界华人消化杂志》1999年第6期504-506,共3页World Chinese Journal of Digestology

摘　　要：目的通过观察外源性ＥＧＦ对大鼠实验性胃溃疡治疗后，对胃粘膜组织学及原癌基因ｃｍｙｃ基因及ｃｆｏｓ基因表达的影响，探讨ＥＧＦ在溃疡治疗中应用的可能性．方法乙酸烧灼法制备溃疡模型．术后次日１５只以西咪替丁１００ｍｇ／（ｋｇ·ｄ）ｓｃ治疗，６只以生理盐水治疗，１４只及１０只对照组大鼠以ＥＧＦ１０μｇ／（ｋｇ·ｄ）ｓｃ治疗，共４ｗｋ．于实验的１ｗｋ及１２ｗｋ分别处死动物，进行组织学及原位杂交检查．结果ＥＧＦ治疗后胃粘膜无异型增生出现．正常胃粘膜ｃｍｙｃ基因表达阴性，ｃｆｏｓ基因表达弱阳性；治疗１ｗｋ后的溃疡边缘ｃｍｙｃ基因、ｃｆｏｓ基因表达弱阳性．实验ｗｋ１２溃疡治愈后胃粘膜ｃｍｙｃ基因表达阴性，ｃｆｏｓ基因表达水平无升高．ＥＧＦ作用正常胃粘膜后，ｃｍｙｃ基因，ｃｆｏｓ基因表达水平无升高．结论外源性ＥＧＦ长期作用于正常及溃疡状态胃粘膜后。AIM To investigate the effect of exogenous epidermal growth factor (EGF) on gastric histology and expression of proto oncogenes c myc and c fos in experimental gastric ulcer in rats. METHODS Experimental gastric ulcer was induced in 35 male Wistar rats with acetic acid. Fourteen rats with gastric ulcer were injected with recombinant human EGF 10μg/(kg·d) sc, and 15 rats with cimetidine 100mg/(kg·d) sc, from the second day for four weeks, six rats treated with normal saline and ten rats with normal gastric mucosa treated with EGF 10μg/(kg·d) sc for 4 weeks served as control. The rats were sacrificed at the end of the first week and 12th week after treatment. c myc and c fos mRNA was detected by in situ hybridization technique using digoxigenin labeled cDNA probe. RESULTS No precancerous lesion appeared after treatment with EGF on experimental gastric ulcer or on normal gastric mucosa in rats. No c myc mRNA was detected in normal gastric mucosa before or after EGF treatment, but low level of c myc mRNA was found in the gastric mucosa near ulcer at the end of the first week in both EGF and cimetidine treated groups. No c myc mRNA was detected in gastric mucosa at the end of the 12th week in both groups when gastric ulcer was healed. Low level of c fos mRNA was found before and after treatment with EGF, but no difference was found between them. CONCLUSION EGF does not cause precancerous lesions or activate pro oncogenes c myc and c fos in gastric mucosa.

关 键 词：胃溃疡 表皮生长因子 c-myc原因 C-FOS基因 

分 类 号：R573.105[医药卫生—消化系统]