共刺激PBLs作用肝癌细胞TCR Vβ基因表达与PTK活性  

TCR V β gene expression and PTK activity of co stimulated PBLs action on hepatoma cells

作　　者：杨红[1] 黄树林[1] 

机构地区：[1]广东药学院分子生物学研究室

出　　处：《世界华人消化杂志》1999年第4期288-290,共3页World Chinese Journal of Digestology

基　　金：国家自然科学基金;广东省科教兴医"五个一"工程重点课题

摘　　要：目的探讨在Ｔ细胞活化信号传递中特异性识别和杀伤肝癌抗原的Ｔ细胞受体（ＴＣＲ）Ｖβ基因亚家族的表达特征，及与蛋白质酪氨酸激酶（ＰＴＫ）活性的关系．方法用单克隆抗体ＣＤ２８＋Ｂ７１（ＣＤ８０）共刺激正常人外周血淋巴细胞（ＰＢＬｓ）后作用于肝癌细胞（ＢＥＬ７４０２），用ＲＴＰＣＲ，Ｓｏｕｔｈｅｒｎ印迹、Ｗｅｓｔｅｒｎ印迹分析ＴＣＲＶβ基因表达特征及ＰＴＫ含量．结果ＴＣＲＶβ７基因在体外选择性扩增，ｄ４表达水平最高，为２４％，对照组为６％，相应地ＰＴＫ的含量也较高，为５８％，对照组为１１％．结论Ｖβ７基因特异性识别肝癌抗原。AIM To study the T cell receptor (TCR) and V β genes subfamilies expression characters in recognizing and killing hepatoma antigen and its relationship to activity of protein tyrosine kinase (PTK). METHODS After monoclonal antibody CD28+CD80(B7.1) co stimulated healthy human peripheral blood lymphocytes (PBLs), and acted on hepatoma cell (BEL 7402), TCR V β7 gene expression characters and PTK activity were analyzed with PT PCR, Southern blot and Western blot. RESULTS TCR V β7 gene was amplified selectively, the expression level was the highest (24%), at day 4 in experimental group, but in the control group, 6%. The PTK was 58% in experimental group, but in the control group, 11%. CONCLUSION V β7 gene is special in recognizing hepatoma antigen, inducing the activation of PTK signal translation.

关 键 词：肝肿瘤 T细胞受体 VΒ基因 基因表达 酪氨酸激酶 

分 类 号：R735.702[医药卫生—肿瘤]