Effects of Dietary β-Glucan and Glycyrrhizin on Non-Specific Immunity and Disease Resistance of the Sea Cucumber (Apostichopus japonicus Selenka) Challenged with Vibrio splendidus  被引量:13

Effects of Dietary β-Glucan and Glycyrrhizin on Non-Specific Immunity and Disease Resistance of the Sea Cucumber (Apostichopus japonicus Selenka) Challenged with Vibrio splendidus

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作  者:CHANG Jie ZHANG Wenbing MAI Kangsen MA Hongming XU Wei 

机构地区:[1]The Key Laboratory, of Mariculture, Ministry of Education of China, Ocean University of China, Qingdao 266003, P. R. China [2]School of Animal Science and Technology, Inner Mongolia University for the Nationalities, Tongliao 028000, P.R. China

出  处:《Journal of Ocean University of China》2010年第4期389-394,共6页中国海洋大学学报(英文版)

基  金:supported by grant No.2006AA100313 from the National High Technology Research and Development Project of P.R. China (863 Project)

摘  要:Sea cucumbers, Apostichopus japonicus Selenka, were fed diets containing non-immunostimulant (basal diet), 0.2% β-glucan and 0.02% glycyrrhizin in a recirculatory water system for 45 days, and subsequently challenged with Vibrio splendidus by injection at 1.0×108 cfu/sea cucumber for 15 days.Phagocytic capacity (PC), intracellular superoxide anion production (ISAP), ly-sozyme (LSZ) activity and superoxide dismutase (SOD) activity in the coelomic fluid were analyzed on the 0th, 5th, 10th and 15th days after injection.Results showed that after the 45-day feeding period, PC, ISAP, LSZ activity and SOD activity in sea cucumbers fed with dietary β-glucan or glycyrrhizin were significantly higher than in those fed with the basal diet.On the 5th day after infection, all the immune parameters examined in the sea cucumbers injected with V.splendidus decreased in value significantly.On the 15th day, PC, ISAP and LSZ activity returned to levels similar to those on the 0th day.For the sea cucumbers injected with saline, there were no significant differences in all the immune parameters examined and in the cumulative morbidity during the 15-day challenging trial.After injecting with V.splendidus, the cumulative morbidity of sea cucumbers fed with the basal diet was significantly higher than those fed with dietary β-glucan or glycyrrhizin when challenged with V.splendidus challenged sea cucumber fed with the basal diet was significantly higher than those fed with dietary β-glucan or glycyrrhizin.There was no significant difference in cumulative morbidity between the dietary β-glucan and glycyrrhizin treatments over time.Sea cucumbers,Apostichopus japonicus Selenka,were fed diets containing non-immunostimulant(basal diet),0.2%β-glucan and 0.02% glycyrrhizin in a recirculatory water system for 45 days,and subsequently challenged with Vibrio splendidus by injection at 1.0×108 cfu/sea cucumber for 15 days.Phagocytic capacity(PC),intracellular superoxide anion production(ISAP),ly-sozyme(LSZ)activity and superoxide dismutase(SOD)activity in the coelomic fluid were analyzed on the 0th,5th,10th and 15th days after injection.Results showed that after the 45-day feeding period,PC,ISAP,LSZ activity and SOD activity in sea cucumbers fed with dietary β-glucan or glycyrrhizin were significantly higher than in those fed with the basal diet.On the 5th day after infection,all the immune parameters examined in the sea cucumbers injected with V.splendidus decreased in value significantly.On the 15th day,PC,ISAP and LSZ activity returned to levels similar to those on the 0th day.For the sea cucumbers injected with saline,there were no significant differences in all the immune parameters examined and in the cumulative morbidity during the 15-day challenging trial.After injecting with V.splendidus,the cumulative morbidity of sea cucumbers fed with the basal diet was significantly higher than those fed with dietary β-glucan or glycyrrhizin when challenged with V.splendidus challenged sea cucumber fed with the basal diet was significantly higher than those fed with dietary β-glucan or glycyrrhizin.There was no significant difference in cumulative morbidity between the dietary β-glucan and glycyrrhizin treatments over time.

关 键 词:Β-GLUCAN GLYCYRRHIZIN Apostichopus japonicus Selenka Immunity Vibrio splendidus 

分 类 号:S831.5[农业科学—畜牧学] S943.116[农业科学—畜牧兽医]

 

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