抑酸对胃黏膜损伤修复时CTGF、EGFR水平的影响  

Effect of acid inhibitors on CTGF and EGFR expression during gastric mucosa repair

作　　者：王健[1] 谭庆华[1] 曾青[1] 

机构地区：[1]贵阳医学院附属医院内镜中心,贵州省贵阳市550004

出　　处：《世界华人消化杂志》2010年第27期2852-2857,共6页World Chinese Journal of Digestology

基　　金：贵州省省长资金资助项目;No.黔省专合字(2007)-67号~~

摘　　要：目的:研究抑酸治疗对电切创面周边组织中结缔组织生长因子(CTGF)、表皮生长因子受体(EGFR)水平的影响.方法:60只SD大鼠,随机分为4组:正常对照组6只,未治疗组18只,雷尼替丁组18只,奥美拉唑组18只.除正常对照组外,对其他动物经腹壁切口及胃窦前壁切口进入胃腔,对胃窦后壁黏膜行电切.术后,各组分别给予雷尼替丁[11mg/(kg·d)]、奥美拉唑[1.8mg/(kg·d)]行尾静脉注射,未治疗组则用等量生理盐水代替.术后1、2、4wk,用免疫组织化学法测定电切创面周边胃黏膜中CTGF及EGFR的变化,并测定IA值.结果:正常组及未治疗组CTGF阳性染色主要在上皮细胞间和黏膜下层的结缔组织中,而雷尼替丁组、奥美拉唑组则主要出现在胃壁细胞内,后两组术后1、2、4wk,CTGF在局部组织中明显增加(4wk,IA:10888±2195,19179±2048vs2370±844,5237±1035,均P<0.05).其中奥美拉唑组在第4周时已明显高于雷尼替丁组(P<0.05).在正常对照组,EGFR阳性染色主要位于胃黏膜浅层壁细胞膜上.术后EGFR主要表达在壁细胞胞浆和黏膜下层的血管内皮细胞的胞浆中.术后1wk,EGFR的表达水平以奥美拉唑组>雷尼替丁组>未治疗组>正常对照组的顺序依次降低(均P<0.05).术后2-4wk,各组均逐渐回落到正常水平(均P>0.05).结论:抑酸治疗有助于胃黏膜电切术后创面的修复,奥美拉唑的修复作用最强,该研究为临床微创治疗后抑酸药的应用以及选择提供了实验依据.AIM：To investigate the effect of acid inhibitors on the expression of connective tissue growth factor（CTGF） and epidermal growth factor receptor（EGFR） during gastric mucosa repair in rats.METHODS：Sixty Sprague-Dawley rats were randomly divided into four groups：normal control group（n = 6）,untreated group（n = 18）,ranitidine group（n = 18）,and omeprazole group（n = 18）.All rats except those in the normal control group underwent gastric mucosal electrocision at the front wall of the antrum.The ranitidine and omeprazole groups were injected with ranitidine [11 mg/（kg·d）] and omeprazole [1.8 mg/（kg·d）] through the caudal vein,respectively,while the normal control group was given equal volume of normal saline.Rats in all the groups were executed at the end of 1,2 and 4wk after operation.The expression of CTGF and EGFR during gastric mucosa repair was determined by immunohistochemistry.RESULTS：In the normal control group and untreated group,positive CTGF signals were located in the lamina propria and submucosal connective tissue.In contrast,positive CTGF signals were mainly located in the cytoplasm of gastric parietal cells in the ranitidine and omeprazole groups.The area and integrated absorbance（IA） of CTGF staining was higher in the ranitidine and omeprazole groups than in the normal control and untreated groups at weeks 1,2 and 4（IA at week 4：10 888 ± 2 195 and 19 179 ± 2 048 vs 2 370 ± 844 and 5 237 ± 1 035,all P 0.05）.The expression level of CTGF in the omeprazole group increased gradually and became higher than that in the ranitidine group at week 4（P 0.05）.Weak EGFR staining was observed in parietal cells in the superfi cial layer of the gastric mucosa in the normal control group.In the untreated group,ranitidine group and omeprazole group,positive EGFR signals were mainly located in the cytoplasm of parietal cells and submucosal vascular endothelial cells.The area and IA of EGFR staining at week 1 were increased markedly in the untreated group,ranitidine

关 键 词：胃黏膜 黏膜切除术 抑酸剂 结缔组织生长因子 表皮生长因子受体 

分 类 号：R573[医药卫生—消化系统]