缺氧诱导因子-1对人脑胶质瘤细胞株T98G放射线敏感性的影响  

Hypoxia Inducible Factor-1 Causes Radioresistance in Human Glioma Cell Line T98G

作　　者：邓瑾[1] 田琪[2] 程国华[1] 张秀萍[1] 苏旭春[1] 

机构地区：[1]广州医学院 附属肿瘤医院,广州510095 [2]天津市儿童医院外科,天津450053

出　　处：《肿瘤预防与治疗》2010年第2期103-106,共4页Journal of Cancer Control And Treatment

摘　　要：目的:观察缺氧诱导因子-1(hypoxia induciblefactory-1,HIF-1)以及其拮抗剂对人脑胶质瘤细胞株T98G放射线敏感性的影响,初步探讨缺氧影响放疗疗效的机制。方法:以化学性缺氧模型观察缺氧诱导因子-1对细胞放射敏感性的影响,以及缺氧诱导因子-1拮抗剂毛壳菌素的放射增敏作用。结果:氯化钴显著降低放射线对T98G细胞的增殖抑制作用,使用毛壳菌素拮抗缺氧诱导因子-1的转录活性可逆转放射线脱敏现象。结论:氯化钴抑制胶质瘤细胞的放射线敏感性,该作用与缺氧诱导因子-1的转录活性相关。Objective： To observe the effect of hypoxia incucible factor-1 （HIF-1 ） on the sensitivity of T98G human glioma cells to radiotherapy and the abrogation of the effect by the antagonist of HIF- 1, hereby provide novel evidence to the radioresistant effect of HIF-1. Methods： Chemical hypoxia model was employed to induce HIF-1 in T98G glioma cells. Proliferation upon the irradiation and/or HIF-1 antagonist was observed by MTT assay. Results： Cobalt chloride desensitized the T98G glioma cells to the proliferation-inhibiting effect of irradiation while the HIF-1 -autagomst chetomin restored this inhibitory effect. Conclusion： HIF-1 plays an essential role in the radioresistance in T98G human glioma cells.

关 键 词：氯化钴 缺氧诱导因子-1 胶质瘤 放射线敏感性 毛壳菌素 

分 类 号：R739.41[医药卫生—肿瘤] R73-354[医药卫生—临床医学]