肺癌患者外周血T淋巴细胞亚群变化特点及临床意义  

The clinical significance of T lymphocyte subsets in peripheral blood from patients with lung carcinoma

作　　者：邹静[1] 刘斌[1] 陈雪华[2] 李宁丽[3] 王利[3] 

机构地区：[1]上海交通大学医学院附属仁济医院呼吸科,上海200127 [2]上海交通大学医学院附属瑞金医院外科,上海200025 [3]上海交通大学医学院上海市免疫学研究所,上海200025

出　　处：《中国免疫学杂志》2010年第11期1016-1020,共5页Chinese Journal of Immunology

基　　金：国家自然科学基金(30670939);仁济医院-基础医学院合作研究基金项目(PY07011)资助

摘　　要：目的:探讨肺癌患者外周血T淋巴细胞变化及其临床意义。方法:采用流式细胞术检测法检测56例肺癌患者(其中20例早中期肺癌、36例晚期肺癌);18例健康人群外周血单个核细胞(Peripheral blood mononuclear cells,PBMC)中总T细胞(CD3+)、Th细胞(CD3+CD4+)、Tc细胞(CD3+CD8+)、NKT细胞(CD3+CD16+CD56+)、NK细胞(CD3-CD16+CD56+)、调节性T细胞(Treg,CD4+CD25+Foxp3+)占CD4+T细胞比例和CD3+γδT细胞比例。结果:56例肺癌患者总T细胞(CD3+)比例明显低于健康组(61.41%±7.88%vs71.63%±5.59%,P<0.001),肺癌患者Tc细胞比例明显低于健康组(23.58%±7.18%vs28.44%±5.20%,P<0.05),肺癌患者Treg比例明显高于健康组(6.20%±1.63%vs3.65%±2.00%,P<0.001);肿瘤组外周血CD3+γδT细胞比例显著低于健康组(3.35%±1.41%vs5.53%±1.87%,P<0.01)。但肺癌患者晚期组与肺癌早中期组比较外周血CD3+γδT细胞比例显著增高(3.70%±1.89%vs2.64%±1.41%,P<0.05),肺癌患者晚期组与早中期组比较外周血Treg细胞比较显著增高(6.78%±2.64%vs5.06%±1.22%,P<0.05)。肺癌患者外周血中NK细胞低于健康组(15.02%±7.61%vs18.74%±6.39%,P<0.05),而Th细胞和NKT细胞都有所降低,但差异尚不具统计学意义(P>0.05)。结论:肺癌患者总T细胞、Th细胞、Tc细胞和NK细胞都有所降低,Treg细胞有所上升,提示肺癌患者处于免疫抑制状态。Treg细胞和CD3+γδT细胞与肺癌的临床病程具有一定的关联性。Objective：To investigate T lymphocyte subsets in peripheral blood from patients with lung carcinoma,and its clinical significance.Methods：The lymphocyte subsets in peripheral blood from 56 lung cancer patients and 18 healthy controls were measured via direct immunofluorescent assay by flow cytometry.The lymphocyte subsets include total T cells（CD3＋）,Th cells（CD3＋CD4＋）,Tc cells（CD3＋CD8＋）,NKT cells（CD3＋CD16＋CD56＋）,NK cells（CD3-CD16＋CD56＋）,regulatory T cells（CD4＋CD25＋Foxp3＋）and CD3＋γδT＋ cells.Results：The amount of total T cells（CD3＋）in the PBMC of the patients with lung cancer was significantly decreased than that of healthy individuals（61.41%±7.88% vs 71.63%±5.59%,P〈0.001）.The ratio of Tc of patients with lung cancer was significantly lower than that of healthy individuals（23.58±7.18% vs 28.44%±5.20%,P〈0.05）.The ratio of Treg（CD4＋CD25＋Foxp3＋）in patients with lung cancer was significantly increased than that of healthy individuals（6.20%±1.635% vs 3.65%±2.00%,P〈0.001）.The ratio of CD3＋γδT＋ cell in PBMC of lung cancer patients was significantly lower than that of healthy individuals（3.35%±1.41% vs 5.53%±1.87%,P〈0.01）.The ratio of NK in PBMC of lung cancer patients was significantly lower than that of healthy individual（15.02%±7.61% vs 18.74%±6.39%,P〈0.05）.The ratio of γδT cells has a significantly statistics difference between the early-middle stage of lung carcinoma and later stage of lung carcinoma（3.70%±1.89% vs 2.64%±1.41%,P〈0.05）.The ratio of Treg cells has a significantly statistics difference between the early-middle stage of lung carcinoma and later stage of lung carcinoma（6.78%±2.64% vs 5.06%±1.22%,P〈0.05）.The amount of Th and NKT cells in PBMC decreased in lung cancer patients,but they didn’t had statistics difference（P〉0.05）.Conclusion：Total T cells,Th,Tc,NK cells in PBMC of the patients with lung cancer decrease and Treg increase.This suggests that the pati

关 键 词：肺癌 T淋巴细胞亚群 调节性T细胞 流式细胞仪 ΓΔT细胞 

分 类 号：R734[医药卫生—肿瘤]