不同金属离子对生物质发酵产氢的影响  

Effects of metallic ions on hydrogen fermentation in mixed anaerobic culture system

作　　者：刘士清[1,2] 刘伟伟[1] 马欢[3] 曹成茂[1] 伍德林[1] 朱德泉[1] 王继先[1] 

机构地区：[1]安徽农业大学工学院,合肥230036 [2]云南师范大学能源与环境科学学院,昆明650092 [3]安徽农业大学生命科学学院,合肥230036

出　　处：《农业工程学报》2010年第10期290-296,共7页Transactions of the Chinese Society of Agricultural Engineering

基　　金：安徽省2010年度第二批科技计划项目(10140702024)

摘　　要：为揭示不同金属离子在生物质发酵产氢中的作用规律,以混合菌群发酵产氢为研究对象,采用正交试验设计的方法,考察了7种金属离子对生物质发酵产氢的影响。通过对不同金属离子质量浓度及相互关系对发酵产氢影响的综合比较,获得以下结论:促进混合菌群发酵产氢的金属离子组成为:Fe2+20mg/L,Zn2+0mg/L,Ni2+1mg/L,Mg2+10mg/L,K+100mg/L,Fe3+100mg/L,Mn2+1mg/L;H2与CO2、总产气量存在显著的正比关系,与糖利用率无直接关系;以PDB(马铃薯水解液)为基质发酵原料,对上述离子组成结果进行验证试验结果表明H2产量和总产气量分别比对照组提高了42.10%和8.06%。研究同时证明:FeSO4对H2的产生有极显著的促进作用,ZnSO4则相反;揭示了Ni2+-Fe3+金属离子对组合可改善发酵产氢条件。该文还从理论上对上述结果进行了分析探讨。An L27（313） orthogonal test was performed in order to investigate the individual and interactive effects of Fe^2＋,Zn^2＋,Ni^2＋,K^＋,Mg^2＋,Mn^2＋,Fe^3＋ seven different metallic ions on biohydrogen production in a mixed anaerobic cultures system.The results showed that hydrogen had prominent direct ratio relationship with carbon dioxide and total gas yield while without glucose utilization ratio.By statistics method,the promoting component to hydrogen production was obtained,which consisted of Fe^2＋ 20 mg/L,Zn^2＋ 0 mg/L,Ni^2＋ 1 mg/L,Mg^2＋ 10 mg/L,K^＋ 100 mg/L,Fe^3＋ 100 mg/L,Mn2＋ 1 mg/L,whereas it was demonstrated that with the promoting component the hydrogen yield and the total gas yield from PDB medium were raised 42.10% and 8.06%,respectively.It was suggested that FeSO4 prominently enhanced the production of hydrogen contrary to ZnSO4 and the couple of Ni^2＋-Fe^3＋ metallic ions was able to activate hydrogenase in the direction of hydrogen production,though optimizing the condition of hydrogen production.Finally,the possible mechanisms underlying the interactive effects of these metallic ions were discussed.

关 键 词：发酵 产氢 金属离子 Ni^2+-Fe^3+金属离子对 

分 类 号：TK6[动力工程及工程热物理—生物能]