微量量热法研究増溶性辅料吐温80对红细胞生理活性的影响  

Microcalorimetric Investigation on Physiological Activity of Red Blood Cells Affected by Excipient of Polysorbate 80

作　　者：张少锋[1,2] 鄢丹[1] 唐慧英[1,2] 冯雪[1,3] 韩玉梅[1] 杨明[2] 王跃生[2] 肖小河[1] 

机构地区：[1]解放军中药研究所解放军302医院,北京100039 [2]江西中医学院,南昌330004 [3]首都医科大学,北京100069

出　　处：《化学学报》2010年第20期2119-2124,共6页Acta Chimica Sinica

基　　金：2007年度中医药行业科研专项(No.200708006);国家"重大新药创制"科技重大专项课题(Nos.2009ZX09502-003;2009ZX09308-005);国家十一五支撑计划课题(No.2008BAI55B04)资助项目

摘　　要：建立灵敏、专属性较好地表征増溶性辅料吐温80对红细胞生理活性影响的评价方法.采用微量量热法以生命体能量代谢特征参数衰亡速率常数(k)、最大热功率(Pmax)、总发热量(Q)、衰亡时间(t)为评价指标,考察增溶性辅料吐温80作用下红细胞的能量动态变化过程.结果表明,随着吐温80浓度(C)的增大,红细胞生长代谢特征参数总发热量(Q)降低、最大热功率(Pmax)增强、衰亡时间(t)减小、衰亡速率常数(k)减小,Q,k与lnC间量效关系良好(相关系数r>0.95);不同厂家吐温80作用于红细胞的总发热量(Q)存在显著性差异(P<0.05).与肉眼观察法、分光光度法、细胞计数等常规方法比较,微量量热法较能实时在线、全息地监测增溶性辅料对红细胞生理活性的动态过程,尤其是对亚致死效应细胞测定的分辨能力.该法可用于增溶性辅料吐温80质量标准的生物测定,亦为红细胞能量代谢等相关研究提供了实验参考.To establish an evaluative method to characterize physiological activity of red blood cells （RBC） affected by polysorbate 80.In the experiment,a microcalorimetric technique was used to investigate kinetic variation of energy metabolism of RBCs,which was evaluated quantitatively by the parameters of decay rate constant （k）,maximum power （Pmax）,the total quantity of heat （Q）,decay time （t） taken from P-t curves.The results indicated that characteristic parameters of k,Q and t decreased,and Pmax increased with the concentration of the polysorbate 80 increasing.Furthermore,there was a good linear relationship between Q,k and ln C （coefficient correlation r〉0.95）,and the total quantity of heat （Q） of two factories had significant difference （P〈0.05）.In contrast with method of macroscopic observation,spectrophotometry and cell counting,microcalorimetry has advantage of real-time,full-scale information in determining the dynamic process of physiological activity of RBCs,especially in measuring sub-lethal cells.It is suggested that this method can be used to bioassay quality of polysorbate 80,and provides reference to related investigation of living cells.

关 键 词：微量量热法 増溶性辅料 吐温80 红细胞 生理活性 

分 类 号：Q25-3[生物学—细胞生物学]