康莱特对胃癌SGC-7901细胞株PPARγ基因的影响  

Effect of Kanglaite on expression of PPAR γ mRNA in gastric cancer SGC-7901 cells

作　　者：陈亦明[1] 徐鲁白[1] 王继生[1] 周斌[1] 余震[2] 

机构地区：[1]温州医学院附属第二医院微创外科,325027 [2]温州医学院附属第一医院普外科

出　　处：《浙江医学》2010年第10期1509-1510,1513,共3页Zhejiang Medical Journal

摘　　要：目的 通过观察康莱特对胃癌SGC-7901细胞株生长以及对过氧化物酶体增殖激活物受体γ（PPAR γ）mRNA表达的影响,探讨康莱特抑制SGC-7901细胞增殖的可能机制.方法 用不同浓度的康莱特处理胃癌SGC-7901细胞,CCK-8法观察细胞增殖抑制作用;RT-PCR法检测胃癌SGC-7901细胞中PPARγ mRNA表达的变化.结果 康莱特抑制SGC-7901细胞增殖,呈浓度和时间依赖性;作用48h后明显抑制胃癌SGC-7901细胞PPAR γ基因表达,呈浓度依赖性.结论 康莱特可能通过下调PPAR γ mRNA的表达来抑制胃癌SGC-7901细胞的增殖.Objective To investigate the effect of Kanglaite on cell growth and expression or peroxlsome proliferators-activated receptor gamma （PPAR γ） in human gastric cancer SGC-7901 cells. Methods SGC-7901 cells were treated with various concentrations of Kanglaite. Cell proliferation was assayed by CCK-8 method and PPAR y mRNA expression was detected by semi-quantitative RT-PCR. Results Kanglaite inhibited the growth of SGC-7901 ceils in a dose-dependent and time-dependent manner. After 48 hours treatment of kanglaite the expression of PPAR γ mRNA in SGC-7901 cells was significantly down-regulated Conclusion Kangiaite can inhibit the growth of SGC-7901 cells, which is associated with a down-regulation of PPAR y mRNA.

关 键 词：康莱特胃癌SGC-7901细胞株 生长抑制 PPARΓ MRNA 

分 类 号：R735.2[医药卫生—肿瘤]