RPC技术检测儿童龈炎中的卟啉单胞菌  

DETECTION OF PORPHYROMONAS GINGIVALIS IN CHILD GINGIVITIS BY MEANS OF THE POLYMERASE CHAIN REACTION

作　　者：金笑一[1] 杨圣辉[1] 张春梅[1] 杨新海[1] 王申五[2] 

机构地区：[1]首都医科大学附属北京口腔医院 [2]北京医科大学附属人民医院

出　　处：《中国微生态学杂志》1999年第2期105-106,共2页Chinese Journal of Microecology

摘　　要：根据牙龈卟啉单胞菌特异的纤毛亚单位蛋白结构基因，设计一对寡核苷酸引物，采用ＰＣＲ扩增了１３１ｂｐ特异片段，实验证明，ＰＣＲ直接检测临床标本中的牙龈卟啉单胞菌，不仅特异、敏感、而且快速，从而显示了较好的优越性。用该引物，分析牙龈卟啉单胞菌在儿童龈炎中的分布。经ＰＣＲ检测４６例龈下菌斑标本中的牙龈卟啉单胞菌，结果表明：２４例标本ＰＣＲ为阳性；对照组４６例标本中，牙龈卟啉单胞菌仅有５例为阳性，儿童龈炎中牙龈卟啉单胞菌的检出率明显高出正常组（ｐ＞０００５）。提示用ＰＣＲ检测儿童龈炎中的牙龈卟啉单胞菌具有重要意义。PCR(Polymerase Chain Reaction)is a new and accurate method in detection of microbiobogy,and is developing rapidly.Primers specific for the fimbrial gene of Porphyromonas gingivalis were selected from the published sequence and used for amplification of a 131-basepair sequence of genomic DNA.In this study,We used PCR for detection of P.g in prepubescent child.92 samples were contained.In child gingivitis group,24 of 46 subgingival bacterial plaque samples contained P.g.In control group,5 of 46 subgingival bacterial plaque samples contained P.g.The difference was significant in statistical analysis(X-test),p<0 005.The study provided a simplified and efficient method for the clinical use.

关 键 词：牙龈 卟啉单胞菌 纤毛 龈炎 儿童 PCR 

分 类 号：R788.3[医药卫生—口腔医学]