普通Taq DNA聚合酶扩增幽门螺杆菌尿素酶基因长片段  

General Taq DNA polymerase amplified long DNA fragments of Helicobacter pylori urease

作　　者：赵庆萱[1] 董勇前[1] 陈翠萍[1] 

机构地区：[1]卫生部兰州生物制品研究所

出　　处：《微生物学免疫学进展》1999年第2期32-34,共3页Progress In Microbiology and Immunology

摘　　要：ＰＣＲ反应扩增较长的ＤＮＡ片段比较困难。本实验对幽门螺杆菌染色体ＤＮＡ上的２７Ｋｂ尿素酶基因用普通ＴａｑＤＮＡ聚合酶进行扩增，扩增结果以琼脂糖凝胶电泳证实，得到所需的２７Ｋｂ的片段。该实验为今后幽门螺杆菌工作的进一步研究提供了经济，有效，简捷的条件。Routine PCR reaction to amplify long DNA fragments is difficult.Our research tried to amplify 2.7 kb urease gene in Helicobacter pylori chromosome DNA using routine Taq DNA polymerase.The agarose gel electrophoresis proved the results that 2.7 kb DNA fragments had been amplified successfully.This experiment provided us a economic,effcient and simple method for later research on Helicobacterpylori urease study.

关 键 词：TAQDNA聚合酶 PCR 幽门螺杆菌 尿素酶 

分 类 号：R378.99[医药卫生—病原生物学]