赤霉素GA_3对小鼠机体氧化-抗氧化系统平衡的影响  

EFFECTS OF GIBBERELLIC ACID (GA_3) ON OXIDATIVE STRESS IN MICE

作　　者：史玉琴[1] 牛翠娇[1] 殷志萍[1] 赵文[1] 

机构地区：[1]河北农业大学食品科技学院,河北保定071001

出　　处：《北京工商大学学报（自然科学版）》2010年第5期32-35,共4页Journal of Beijing Technology and Business University:Natural Science Edition

基　　金：河北农业大学新兴学科科研发展项目(FSY200563)

摘　　要：选择昆明种小鼠为试验动物,以超氧化物歧化酶、谷胱苷肽过氧化物酶活性及丙二醛含量为观察指标,研究赤霉素GA3在剂量(197,394和788 mg/kg)暴露条件下,小鼠脾脏、肝脏和心脏组织是否发生氧化应激.结果表明,394和788 mg/kg每千克体重bw剂量组脾脏超氧化物歧化酶、谷胱苷肽过氧化物酶活性有所下降,与对照组相比,分别降低了21.01%,29.02%和23.36%,35.03%,与对照组相比差异显著;肝脏超氧化物歧化酶、谷胱苷肽过氧化物酶活性分别降低了9.01%,13.05%和12.81%,16.17%,与对照组相比差异显著(P<0.05,P<0.01).同时赤霉素GA3处理后丙二醛含量显著升高,394,788 mg/kg bw剂量处理组脾脏和肝脏分别升高了19.06%,23.61%和22.33%,26.39%,与对照组相比差异显著(P<0.05,P<0.01);肾脏超氧化物歧化酶、谷胱苷肽过氧化物酶活性有所上升,与对照组相比,分别上升了22.34%和40.20%,丙二醛含量有所下降,与对照组相比分别下降了35.93%和30.05%;另外,赤霉素GA3处理后,对心脏的氧化应激作用与对照组相比差异不显著.表明赤霉素对小鼠不同组织器官的氧化应激表现不同,对肝脏、脾脏有氧化损伤作用,对心脏作用不明显.The effect of Gibberellic acid（GA3） on oxidative stress in Mice was investigated.The activity of superoxide dismutase（SOD）,glutathione peroxidase（GSH-Px） and the contents of malondialdehyde（MDA） in spleen,liver and heart were determined.The results showed that the activity of SOD and GSH-Px was significantly lower than those in the control group（P0.05,P0.01）,which decreased by 21.01%,29.02%,and 23.36%,35.03%,respectively,at 394 and 788mg/kg bw dose levels in the spleen;and decreased by 9.01%,13.05% and 12.81%,16.17%,respectively,at the same dose levels in the liver.The contents of MDA were remarkably higher than those in the control group at the same dose levels（P0.05,P0.01）,which increased 19.06% and 23.61%,respectively,at 394 and 788mg/kg bw dose levels in the spleen;and increased 22.33%,26.39%,respectively,at the same dose levels in the liver.The activity of SOD and GSH-Px was higher than those in the control group in the kidney,which increased 22.34% and 40.20%.And the contents of MDA were lower than those in the control group（P0.05,P0.01）,which decreased 35.93% and 30.05%.But it caused unconspicuous alterations in the heart.The results suggested that Gibberellic acid（GA3） might produce different oxidatie stress in mice＇s various tissues.It caused a significant oxidative damage in the spleen and liver,but it caused an unconspicuous oxidative damage in the heart.

关 键 词：赤霉素 小鼠 抗氧化酶 丙二醛 氧化应激 

分 类 号：TS201.6[轻工技术与工程—食品科学]