致病性犬瘟热病毒CDV-JT1株的分离与鉴定  

Isolation and identification of pathogenic canine distemper virus CDV-JT1 strain

作　　者：王秀东[1] 谭斌[1] 陈微晶[2] 陈立志[1] 武华[1,2] 

机构地区：[1]中国农业科学院特产研究所,吉林吉林132109 [2]北京必威安泰生物科技有限公司,北京100176

出　　处：《中国兽医学报》2010年第11期1413-1417,共5页Chinese Journal of Veterinary Science

基　　金：国家科技支撑计划资助项目(2009BADB4B02)

摘　　要：应用MDCK细胞从吉林某地犬瘟热（Canine distemper,CD）疑似发病犬肺脏组织中分离出1株病毒,该分离毒株经MDCK细胞传至第6代时出现典型的合胞体细胞病变（CPE）。经病毒形态观察、理化特性鉴定、RT-PCR和直接免疫荧光鉴定可知该分离毒株为犬瘟热病毒（CDV）,命名为CDV-JT1;动物试验表明,试验犬接种CDV-JT1后21 d内都出现典型的犬瘟热症状;H基因序列分析表明,CDV-JT1株的H基因与中国分离株CDTaiChung株、TN株、SHLJ（07）1株、日本Hamamatsu株、Ueno株、Yanaka株和KDK-1株在基因型上同属于Asia-1型。CDV-JT1株含有包括CDV野毒株特有的309~311位在内的8个潜在的N-连接天冬酰氨糖基化位点。CDV-JT1强毒株的分离成功,对进一步开展疫苗研发、流行病学调查以及致病机理等方面的研究具有重要意义。A virus strain was isolated by MDCK cells from lung of a dog with suspected CDV infection.The isolate could induce cytopathogenic effects of syncytia in MDCK cells after six passages.It was demonstrated to be CDV by morphology,physical and chemical properties,RT-PCR and direct immunofluorescent assay（FA） and was named CDV-JT1.Dogs artificially infected by CDV-JT1 developed symptom of CD during 21 days after infection.The H gene of the virus was sequenced and showed that the isolate,together with CDTaiChung,TN,SHLJ（07）1,Hamamatsu,Ueno,Yanaka and KDK-1 strains,belonged to the Asia-1 type CDV.CDV-JT1 strain contained eight protential asparagines glycosylation sites of which the site of 309-311 was unique for wild CDV strains.The successful isolation of CDV-JT1 provided a basis for futher search on vaccination,epidemiology,pathogenicity.

关 键 词：犬瘟热病毒 强毒株 分离和鉴定 H基因 

分 类 号：S852.65[农业科学—基础兽医学]