2种纤维素酶水解效果比较及酶蛋白组分分析  

Comparison between two kinds of cellulases in enzymatic hydrolysis and their secreted proteins analysis

作　　者：连之娜[1] 李鑫[1] 欧阳嘉[1] 陈牧[1] 勇强[1] 余世袁[1] 

机构地区：[1]南京林业大学林木遗传与生物技术省部共建教育部重点实验室,南京210037

出　　处：《生物加工过程》2010年第6期57-62,共6页Chinese Journal of Bioprocess Engineering

基　　金：国家重点基础研究发展计划(973计划)资助项目(2007CB707801);国家高技术研究发展计划(863计划)资助项目(2006AA020204)

摘　　要：比较了自产纤维素酶和商品纤维素酶的水解效果,并采用超滤、层析、SDS-PAGE相结合的方法分析2种纤维素酶蛋白组分的差异。里氏木霉以纸浆为C源合成的自产纤维素酶的水解得率高于商品纤维素酶,自产纤维素酶水解48h的得率为66.24%,商品纤维素酶的得率为52.19%。自产纤维素酶中存在着Cel6A酶组分和XYNⅡ酶组分,而商品纤维素酶中没有检测到这2种酶组分。自产纤维素酶和商品纤维素酶的Cel1A酶组分和Cel7A酶组分间存在着分布和含量上的差异。自产纤维素酶在相对分子质量(2.5～3.5)×104范围内存在着几条蛋白条带,而商品纤维素酶则是在相对分子质量3.5×104附近存在着几条蛋白条带。The enzymatic hydrolysis effect was evaluated between Trichoderma reesei（T.reesei）cellulase produced in this laboratory and commercial cellulase.Differences of secreted proteins＇ profiles from the two cellulases were investigated by the operations of ultrafiltration,chromatography,and SDS-PAGE.The higher enzymatic hydrolysis yield was obtained with the enzyme from T.reesei.The enzymatic hydrolysis yield was 66.24% from T.reesei cellulase,and 52.19% from commercial cellulase for 48 h.Cel 6A and XYNⅡ could be found in T.reesei cellulase but absent in commercial cellulase.Cel 1A and Cel 7A were different in both in distribution and the amount between T.reesei cellulase and commercial cellulase.Several protein bands were detected in the MW of（2.5-3.5）×104 of T.reesei cellulase while some protein bands were displayed in the MW around 3.5×104 in commercial cellulase.

关 键 词：纤维素酶 里氏木霉 纤维素水解 

分 类 号：TQ351[化学工程]