酿酒葡萄原生质体再生植株  

Plant Regeneration From Protoplasts of Wine-making Grapevine

作　　者：于向荣 李佩芬 卢炳芝 王敏 郑旭琴 刘延松 

机构地区：[1]山东省酿酒葡萄科学研究所,济南250100

出　　处：《果树科学》1999年第2期115-118,共4页Journal of Fruit Science

摘　　要：将酿酒葡萄品种白诗南、梅郁的花丝接种在含６ＢＡ２．０ｍｇ·１－１、２，４－Ｄ０．５ｍｇ·１－１的Ｂ５诱导培养基上，诱导产生胚性愈伤组织。胚性愈伤组织经液体悬浮培养形成含大量胚性细胞团的悬浮培养物。用Ｃｅｌｌｕｌａｓｅ　　Ｒｓ２％、Ｐｅｃｔｏｌｙａｓｅ　Ｙ２３０．３％，５ｍｍｏｌ·１－１ＣａＣｌ２·２Ｈ２Ｏ、０．６ｍｏｌ·１－１甘露醇、０．３％葡聚糖硫酸钾、ｐＨ５．６～５．８的酶混合液从胚性细胞团分离得到原生质体。原生质体培养到第５天出现第一次分裂，４０ｄ形成上百个细胞的大细胞团，５０ｄ形成０．５～１．０ｍｍ大小的小愈伤组织。这些小愈伤组织在含６ＢＡ０．５ｍｇ·１－１的Ｂ５分化培养基上分化出胚状体进而形成幼苗，在生根培养基上生根形成再生植株。The embryogenic calli were produced from the filaments of Chenin Blanc and Met Yu cultured on B, medium with 0. 5~ 1 mg. l-1 2,4-D, 2~3 mg. l -1 6BA. The high percentage embryogenic cell colonies were established from the calli shaking in B5 liquid medium with 0.5ing.l -1,4-D,0.01 mg. l -1 NAA. The cell suspension culture was used for protoplasts isolation. Protoplastswere obtained with emzyme mixture containing 2 % Cellulade Rs, 0. 3 % Pectolyase Y23,5 mol.l-1 CaCl2.2H2O, 0. 6 mol.l -1 mannital, 0. 3 % dextran sulfate potassium salt at pH 5. 6~5. 8.The protoplasts cultured in B, liquid medium with 0. 5 mg. l -12, 4 -D entered division after fivedays and formed colonies with hundreds cells about forty days. The small calli of 0. 5~1. 0 mmwere formed about fifty days. When it was transferred to B5 liquid medium supplemented with6BA 0.5mg. l -1, the small calli differentiated into embroids, then developed into plantlet on rootmedium with 0. 2 mg. l -1 IAA,0. 2 mg. l -1 IBA,0. 3mg. l -1 6BA.

关 键 词：酿酒葡萄 原生质体 再生植株 葡萄 

分 类 号：S663.1[农业科学—果树学]