Production and Recovery of Rhamnolipids Using Sugar Cane Molasses as Carbon Source  

作　　者：Ana Carmen Santos Marcio Silva Bezerra Heloize dos Santos Pereira Everaldo Silvino dos Santos GoreteRibeiro de Macedo 

机构地区：[1]Federal University of Rio Grande do Norte, Center of Technology, Chemical Engineering Department, Biochemical Engineering Laboratory, Campus Universitario, Lagoa Nova, 3000, CEP.＇59072-970 Natal, Rio Grande do Norte, Brazil

出　　处：《Journal of Chemistry and Chemical Engineering》2010年第11期27-33,共7页化学与化工（英文版）

摘　　要：Biosurfactants were synthesized by Pseudomonas aeruginosa （P.A.）, using sugar cane molasses as carbon source. Assays were conducted in a shaker with agitation speed of 200 rpm, temperature of 38 ℃ and aeration ratio （Vm/Vf） of 0.4 and 0.6. A concentration of 3.0% was used for the carbon and energy source （molasses） and of 0.3% for the nitrogen source （NaNO3）. Samples were removed at regular times until 96 hours of cultivation. The reduction in surface tension was measured using the ring method; cell concentration was obtained by the dry mass and substrate consumption by the DNS method. The metabolite produced was extracted and quantified by the thioglycolic method. The results showed a maximum surface tension reduction of 46.57% after 60 h, 3.63 g/L of biomass after 8 h （μXmax =0.15 h^-1）, 79.60% of substrate consumption （μs= 0.67 h-1） and 4.47 g/L of rhamnolipid （μp=0.029 h^-1）.

关 键 词：Biosurfactants MOLASSES RHAMNOLIPIDS Pseudomonas aeruginosa. 

分 类 号：TQ929[轻工技术与工程—发酵工程] TS249.3