影响动物细胞有丝分裂方向的因素应力纤维在纺锤体定位中的作用  

The Role of Stress Fiber in Spindle Positioning Affects the Orientation of Animal Cells Mitosis

作　　者：赵磊[1] 杨春[2] 庄逢源[1] 

机构地区：[1]北京航空航天大学生物与医学工程学院,北京100191 [2]青华大学航天航空学院,北京100084

出　　处：《中国生物化学与分子生物学报》2010年第11期981-987,共7页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家自然科学基金(No.30870602,No.30671098)资助项目~~

摘　　要：细胞的有丝分裂与细胞的增殖、分化及胚胎发育、组织器官形成、损伤组织的修复和疾病的发生有关.各种物理因素、细胞所处的微环境(包括细胞外基质、细胞粘附)等,以及胞内的多种信号因子均能对细胞的有丝分裂方向产生影响.大量文献表明,应力纤维的排列为有丝分裂中心粒分离和定位提供轨道,最终影响纺锤体和有丝分裂的定向.本实验室的micro-pattern和静态单轴拉伸应变实验进一步提示了应力纤维的排布方式是影响有丝分裂方向的重要因素.本文围绕着应力纤维的排布对有丝分裂方向的影响这一研究观点,综述分析了整合素介导的细胞外粘附-黏着斑的组装-应力纤维的排布-有丝分裂纺锤体定向等一系列影响贴壁哺乳动物细胞有丝分裂定向的过程.并根据酵母模型,对哺乳动物细胞有丝分裂定向过程的分子机制进行了介绍;在该过程中肌球蛋白、动力蛋白和kar9等蛋白质起到重要作用.The cell orientation during mitosis is important for cell proliferation,differentiation,embryonic development,organogenesis,tissue injury recovery and disease generation.The factors in intracellular and extracellular microenvironment,such as extracellular matrix,cell adhesion,signal protein,etc.,may impact on the mitosis orientation.It is known that stress fibers form a scaffold to migrate the centrosomes to opposite sides and determines the spindle orientation of mitosis.An correlation between stress fiber alignment and mitosis orientation was also demonstrated by micro-pattern and uniaxial static stretch experiments.We reviewed a model of integrin-mediate extracellular adhesion—focal adhesion assembly—stress fiber alignment—spindle positioning that determined the division orientation of adherent mammalian cells.The involved factors suggested from the data in yeasts model,such as myosin,dynein and kar9,were discussed for their potential roles.

关 键 词：哺乳动物细胞 有丝分裂 应力纤维 不对称分裂 

分 类 号：Q2[生物学—细胞生物学] Q66