球囊损伤大鼠主动脉内皮后血管紧张素Ⅱ2型受体的表达及瑞舒伐他汀的干预作用  

Expression of AngiotensinⅡ Type 2 Receptor and the Effects of Rosuvastatin on it After Vascular Balloon Injury in Rats

作　　者：刘平[1] 李永红[1] 苘瑜琳[1] 蔡尚郎[1] 

机构地区：[1]青岛大学医学院附属医院心内科,山东省青岛市266003

出　　处：《中国动脉硬化杂志》2010年第9期709-712,共4页Chinese Journal of Arteriosclerosis

基　　金：青岛市科学技术局资助(02-2-kj-yn-25)

摘　　要：目的通过观察血管内皮损伤后血管紧张素Ⅱ2型受体表达的变化及瑞舒伐他汀对其表达的影响,探讨血管紧张素Ⅱ2型受体在再狭窄中的作用。方法建立球囊损伤大鼠主动脉内皮模型,并将大鼠随机分为对照组、手术组和瑞舒伐他汀组,每组分别于术后14天、28天取主动脉组织,并行HE染色观察血管内皮厚度的变化,用逆转录聚合酶链反应检测主动脉组织血管紧张素Ⅱ2型受体mRNA的表达,免疫组织化学法检测主动脉组织血管紧张素Ⅱ2型受体蛋白的表达。结果大鼠主动脉球囊损伤术后14天及28天血管内皮较对照组显著增厚(P<0.01),瑞舒伐他汀治疗14天及28天大鼠主动脉内膜增生较手术组明显减轻。术后14天及28天,手术组血管紧张素Ⅱ2型受体mRNA和蛋白表达较对照组显著升高(P<0.05),瑞舒伐他汀组血管紧张素Ⅱ2型受体mRNA和蛋白表达较手术组增加(P<0.05)。结论瑞舒伐他汀可抑制大鼠主动脉内皮损伤后的血管增生,并上调血管内皮血管紧张素Ⅱ2型受体的表达。Aim To investigate the expression of angiotentionⅡ type 2 receptor（AT2） and the effect of rosuvastatin on it after vascular balloon injury in rats. Methods Rat models of aortic endothelial denudation were established by 2F balloon catheters.Rats were randomly allocated into three groups： control group,vascular balloon injury group and rosuvastatin treatment group.The expression of AT2 mRNA and protein were investigated at day 14 and 28 after injury by reverse transcription-polymerse chain reaction（RT-PCR） technique and immunohistochemistry method,respectively. Results Significant intimal thickening was observed at 14 days and 28 days after injury.Rosuvastatin significantly prevented intimal thickening at 14 days and 28 days after balloon injury.The expression of AT2 mRNA and protein increased significantly at 14 days and 28 days after injury（P〈0.05）,and they increased obviously after rosuvastatin treatment（P〈0.05）. Conclusion The expression of AT2 mRNA and protein increased significantly after endothelial injury,and rosuvastatin upregulated the expression of AT2.

关 键 词：瑞舒伐他汀 球囊损伤 血管紧张素Ⅱ2型受体 

分 类 号：R363[医药卫生—病理学]