电针“三阴交”穴对痛经大鼠子宫丙二醛、β-内啡肽含量及热休克蛋白70表达的影响  

Effects of Electroacupuncture of "Sanyinjiao"(SP 6) on Contents of MDA and β-EP,and Expression of HSP 70 in the Uterus in Rats with Dysmenorrhea

作　　者：宋晓琳[1] 张露芬[1] 李晓泓[1] 徐莉莉[1] 李春华[1] 丁喜艳[1] 任晓暄[1] 赵雅芳[1] 郭孟玮[1] 孙志芳[1] 朱江[1] 

机构地区：[1]北京中医药大学针灸推拿学院,北京100029

出　　处：《针刺研究》2010年第5期342-346,共5页Acupuncture Research

基　　金：国家"973"课题(No.2006CB504503);国家中医药管理局针灸特色疗法评价重点实验室课题

摘　　要：目的:观察电针"三阴交"对痛经大鼠子宫的保护作用,分析其镇痛作用的机制。方法:通过阴道涂片法,筛选出处于动情间期的3月龄SD雌性大鼠48只,以苯甲酸雌二醇和缩宫素制造痛经模型。按照随机数字表法将大鼠分为盐水组、模型组、电针组(造模结束后电针双侧"三阴交"穴20 min),每组16只。采用放射免疫法检测大鼠子宫丙二醛(MDA)、β-内啡肽(-βEP)含量,免疫组化法检测子宫热休克蛋白70(HSP70)的表达。结果:与盐水组比较,痛经模型组大鼠子宫MDA含量显著升高(P<0.01),子宫-βEP含量显著降低(P<0.01),子宫HSP 70表达有降低趋势。与模型组比较,电针组大鼠子宫MDA含量明显降低(P<0.01),子宫-βEP含量显著升高(P<0.01),并有升高痛经模型大鼠子宫HSP 70表达的趋势。结论:电针"三阴交"穴可以清除痛经大鼠子宫的氧自由基,增加子宫局部镇痛物质-βEP,这可能是其对子宫保护和镇痛作用的机制之一。Objective To investigate the effect of electroacupuncture（EA） of ＂Sanyinjiao＂（SP 6） on the uterus in dysmenorrhea rats so as to study its underlying analgesic mechanism.Methods A total of 48 SD rats during diestrus were randomized into normal saline（control） group,model group and acupuncture group according to a random number table,with 16 rats in each group.Dysmenorrhea model was established by subcutaneous injection of Estradiol benzoate（0.5 mg/d on the 1st and 10th day,and 0.2 mg/d from day 2 to day 9,once daily for 10 days） and oxytocin（2 U/rat,once on day 10）.Malondialdehyde（MDA） and β-endorphin（β-EP） contents in the uterus were detected by radioimmunoassay,and the heat shock protein 70（HSP 70） immunoactivity of the uterus was detected by immunohistochemistry.Results In comparison with the control group,MDA content in the uterus was increased significantly in the model group（P0.01）,while the β-EP level and the immunoactivity of HSP 70 immuno-reaction（IR） positive products in the uterus decrease significantly（P0.01）and moderately,respectively in the model group.In comparison with the model group,uterine MDA content in the EA group was decreased significantly（P0.01）,while uterine β-EP level increased considerably（P0.01）and HSP 70 expression was upregulated to a certain degree.Conclusion EA of ＂Sanyinjiao＂（SP 6） can reduce MDA content and upregulate β-EP level of the uterus in rats with dysmenorrhea,which may contribute to its analgesic effect in relieving dysmenorrhea by clearing away oxygen free radicals and raising analgesic substance in the uterus.

关 键 词：电针 三阴交穴 痛经 子宫 丙二醛 β-内啡肽 热休克蛋白70 

分 类 号：R771.51[医药卫生—眼科]