表没食子儿茶素没食子酸酯对结肠癌皮下易位的原位荧光动物模型肿瘤生长和转移的作用  

Epigallocatechin-3-gallate suppresses subcutaneous tumor growth in an orthotopic transplantation mouse model of colorectal cancer derived from cells expressing enhanced green fluorescent protein

作　　者：王水明[1] 金黑鹰[2] 张春霞[1] 孙宇 杨志坚 丁义江[2] 

机构地区：[1]南京中医药大学研究生部,江苏省南京市210068 [2]全国肛肠医疗中心南京中医药大学第三附属医院,江苏省南京市210001 [3]南京源端生物科技公司,江苏省南京市210003

出　　处：《世界华人消化杂志》2010年第28期3016-3019,共4页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.30572447;No.30973837;江苏省自然科学基金资助项目;No.BK2007009~~

摘　　要：目的:研究表没食子儿茶素没食子酸酯(EGCG)在结肠癌皮下易位的原位荧光动物模型中,对结直肠癌的生长、转移的作用.方法:采用人结肠癌HT-29建立裸鼠的皮下瘤模型,4wk后进行皮下瘤原位移植,建立结肠皮下易位的结肠癌荧光原位移植模型.39只荷瘤裸鼠分为生理盐水对照组和5、10、20mg/kgEGCG组.EGCG灌胃治疗14wk后,在荧光影像系统下观察原发肿瘤以及有无转移情况,并计算肿瘤的体积.结果:使用5、10和20mg/kg3个剂量EGCG治疗后,在第7、14、26和35天,治疗组肿瘤生长速度明显低于对照组肿瘤生长速度,20mg/kgEGCG组治疗第26天肿瘤体积明显小于对照组,差异具有统计学意义(106.7mm3±155.5mm3vs393.3mm3±361.1mm3,P<0.05);3个治疗组之间比较,差异无统计学意义.结论:EGCG对结肠癌皮下原位荧光动物模型肿瘤生长有明显的抑制作用,其作用效果与作用时间成正相关.AIM：To investigate the anticancer effect of epigallocatechin-3-gallate （EGCG） in an orthotopic transplantation mouse model of colorectal cancer derived from cells expressing enhanced green fluorescent protein （eGFP）.METHODS：The human colon cancer cell line HT-29 expressing eGFP was used to establish a subcutaneous tumor model of colorectal cancer in nude mice.Four weeks later,the subcutaneous tumors were subjected to orthotopic transplantation to establish an orthotopic transplantation mouse model of colorectal cancer.A total of 39 model mice were obtained and divided into four groups：a control group receiving saline and three treatment groups receiving different doses of EGCG （5,10 and 20 mg/kg,respectively） for 14 d.Tumor metastasis was then observed and tumor volume was calculated using a fluorescence imaging system.RESULTS：At 7,14,26 and 35 d after treatment with different doses of EGCG,tumor growth velocity was significantly lower in all the treatment groups than in the control group.Tumor volume was significantly smaller in the EGCG （20 mg/kg） treatment group than in the control group （106.7 mm 3±155.5 mm 3 vs 393.3 mm 3±361.1 mm 3,P0.05）.There were no significant differences in tumor volume among the three treatment groups.CONCLUSION：EGCG can significantly inhibit tumor growth in an orthotopic transplantation mouse model of colorectal cancer in a timedependent manner.

关 键 词：茶提取物 表没食子儿茶素没食子酸酯 结直肠癌 在体研究 原位移植模型 

分 类 号：R285.5[医药卫生—中药学]