家蚕蛹和成虫期GOBP/PBP亚家族基因簇基因定位与表达分析  

Mapping and expression analysis of GOBP/PBP subfamily gene cluster during pupal and adult stages of the silkworm,Bombyx mori

作　　者：张升祥[1] 张瑶[1] 徐世清[2] 王更先[3] 胡增娟 赵春晓[1] 崔为正[1] 

机构地区：[1]山东农业大学林学院,山东泰安271018 [2]苏州大学基础医学与生物科学学院,江苏苏州215123 [3]邯郸学院生物科学系,河北邯郸056005 [4]青岛市农业广播电视学校,山东青岛266071

出　　处：《昆虫学报》2010年第10期1069-1076,共8页Acta Entomologica Sinica

基　　金：现代农业产业技术体系建设专项资金

摘　　要：昆虫的气味结合蛋白(odorant binding proteins,OBPs)在昆虫与外界环境化学信息交流过程中起着重要作用,对昆虫觅食、求偶、繁殖具有重要意义。普通气味结合蛋白/性信息素结合蛋白(general odorant binding protein/pheromone binding protein,GOBP/PBP)是鳞翅目昆虫OBP家族的一个重要单系群。为进一步明确家蚕Bombyx moriGOBP/PBP基因的结构、表达及功能,本研究利用染色体定位及半定量表达分析方法对其进行了分析。染色体定位分析显示,这些基因以基因簇的形式存在于第19染色体的nscaf3052上,基因结构相似,转录方向一致,表明这些基因可能由同源基因复制产生,并具有类似功能。对家蚕蛹和成虫不同发育阶段的雌、雄虫多种组织中进行表达分析发现,这些基因的表达在不同发育时期和不同组织间差异明显(P<0.05),相对表达量均以触角中为最高,其他非嗅觉组织中也多有表达,性别间差异不大,说明了该基因簇基因除了具有嗅觉相关的功能外,很可能具有其他尚未被发现的功能。Odorant binding proteins （OBPs） in insects play a primordial role in foraging,courtshipping,multiplying,and chemical communication with the environments.General odorant binding protein/pheromone binding protein （GOBP/PBP） is an important monophyletic group of Lepidoptera OBP family.We analyzed the GOBP/PBP in the silkworm Bombyx mori,the model organism of Lepidoptera,using chromosome mapping and semi-quantitative RT-PCR for further understanding of the expression and function of GOBP/PBP.Chromosome mapping showed that these genes were organized in the nscaf3052 of chromosome 19 in the form of gene cluster,with similar gene structure and identical transcription direction,suggesting that they were originated from the duplication of homologous genes and probably possess functional similarity.Analysis of the expression profiles in different tissues of males and females during pupal and adult stages revealed that these genes possessed conspicuous temporal and spatial expression differences （P0.05）,the highest relative expression level occurred in the antenna,and lower relative expression levels in other most non-olfactory tissues without significant sex differences.The results suggest that these genes may have other functions undiscovered besides olfactory-related function.

关 键 词：家蚕 气味结合蛋白 PBP1-GOBP2亚家族 基因簇 表达分析 染色体定位 

分 类 号：Q966[生物学—昆虫学]