光肩星天牛体内纤维素酶活性及其与寄主选择的关系(英文)  

Cellulase activity and its relationship with host selection in the Asian longhorned beetle,Anoplophora glabripennis(Coleoptera:Cerambycidae)

作　　者：李晓娟[1] 阎雄飞[2] 骆有庆[1] 田桂芳 年寅杰 张铁林 

机构地区：[1]北京林业大学省部共建森林培育与保护教育部重点实验室,北京100083 [2]榆林学院,陕西榆林719000 [3]吴忠市园林局,宁夏吴忠751100

出　　处：《昆虫学报》2010年第10期1179-1183,共5页Acta Entomologica Sinica

基　　金：supported by the National Natural Science Foundation of China ( Grant No.30571503);the Program for Changjiang Scholars and Innovative Research Team in Universities (PCSIRT0607)~~

摘　　要：为了揭示光肩星天牛Anoplophora glabripennis Motschulsky纤维素酶与寄主选择的关系,以4种不同寄主树种(新疆杨Populusalba var.pyramidalis Bunge、箭杆杨Populusnigra var.thevestina(Dode) Bean、合作杨Populus simonii×Populus pyramidalis cv.opera Hsu和漳河柳Salixmatsudana f.lobato-glandulosa Fauget Liu来源的光肩星天牛幼虫,和以取食5种不同树种(臭椿Ailanthus altissima、毛白杨Populus tomentosa、合作杨、旱柳Salixmatsudana Koidz.和复叶槭Acernegundo Linn.)的光肩星天牛成虫为实验对象,测定其纤维素酶活性。结果表明:不同寄主来源的光肩星天牛幼虫内切-β-1,4-葡聚糖酶活性在1.36～2.71μmol葡萄糖/(gFW.h)之间,β-葡萄糖苷酶活性在2.57～4.86μmol葡萄糖/(gFW.h)之间;取食不同树种的光肩星天牛成虫内切-β-1,4-葡聚糖酶活性在4.08～9.27μmol葡萄糖/(gFW.h)之间,β-葡萄糖苷酶活性在2.87～6.08μmol葡萄糖/(gFW.h)之间。不同寄主树种来源的光肩星天牛幼虫体内纤维素酶活性无显著性差异,取食与否以及取食树种的不同对光肩星天牛成虫纤维素酶活有较大影响。To investigate the relationship between cellulase activity and host selection,Anoplophora glabripennis （Motsch.） larvae from four different host trees （Populus alba var.pyramidalis Bunge,Populus nigra var.thevestina （Dode） Bean,Populus simonii × Populus pyramidalis cv.opera Hsu and Salix matsudana f.lobato-glandulosa Fang et Liu） and adults fed on five different tree species were selected.The enzyme activities of endoglucanase and β-glucosidase in the intestines of the insects were measured.The results showed that in the larvae,the activity of endoglucanase ranged from 1.36 to 2.71 μmol glucose/（g FW·h）,and the activity of β-glucosidase ranged from 2.57 to 4.86 μmol glucose/（g FW·h）.In the adults fed on different tree species,the activity of endoglucanase ranged from 4.08 to 9.27 μmol glucose/（g FW·h）,and the activity of β-glucosidase ranged from 2.87 to 6.08 μmol glucose/（g FW·h）.There are no significant differences in cellulase activities among the larvae from different host trees.However,the tree species which the adults fed upon has great effect on the activity of cellulase in the adults.

关 键 词：光肩星天牛 消化酶 纤维素酶 酶活性 寄主选择 

分 类 号：Q965[生物学—昆虫学]