外周血泌乳素水平与T淋巴细胞亚群的相关性研究  

The study of the relevance of PRL and T-lymphocyte subsets in the peripheral blood

作　　者：于晶[1] 陈镭[1] 苏治国[1] 刘洪良[1] 赵玉军[1] 刘振林[1] 

机构地区：[1]天津市第五中心医院脑系科,300450

出　　处：《中国实用医药》2010年第33期3-5,共3页China Practical Medicine

摘　　要：目的本通过研究外周血PRL水平与T淋巴细胞亚群变化的相关性,以探讨泌乳素(PRL)与免疫系统变化的关系。方法检测100例垂体腺瘤患者及56例体检健康人外周血中CD4+CD25+、CD3+、CD3+CD4+及CD3+CD8+的T淋巴细胞水平;并测定其外周血中PRL水平。结果病例组外周血CD3+T淋巴细胞显著低于健康人组(P<0.0001);外周血CD3+CD4+T淋巴细胞及CD3+CD8+T淋巴细胞病例组与健康人组均无显著差异(P>0.05);病例组外周血CD4+CD25+T淋巴细胞为显著高于健康人组(P<0.0001)。其中病例组中PRL<100ng/ml组外周血CD3+T淋巴细胞及CD3+CD4+T淋巴细胞均显著低于PRL>100ng/ml组(P<0.05);外周血CD3+CD8+T淋巴细胞及CD4+CD25+T淋巴细胞,病例组与健康人组均无显著差异(P>0.05)。结论 CD3+T淋巴细胞的减少可能是垂体肿瘤细胞本身刺激CD4+CD25+T淋巴细胞的增生,导致外周血IL-2减少,从而抑制了T淋巴细胞的活化和增殖。外周血PRL>100ng/ml组外周血PRL显著升高,通过刺激各种细胞因子的分泌,而进一步促进T淋巴细胞的活化及增殖,同时,外周血中高PRL可直接抑制外周血T淋巴细胞的凋亡。Objective To investigate the relationship between PRL and immune system,the level of PRL and the changes of T-lymphocyte subsets were detected in the peripheral blood.The relevance of them was also analyzed.Methods 100 patientents with pituitary adenoma and 56 people were used.The peripheral blood of every person was collected for determining the levels of T-lymphocyte subsets of CD4 ＋ CD25 ＋,CD3 ＋,CD3 ＋ CD4 ＋ and CD3 ＋ CD8 ＋ with flow cytometry.The levels of PRL in peripheral blood were omit measured at the same time with the method of magnetic separation enzyme immunoassay as well.Results The amount of CD3 ＋ lymphocytes in the peripheral blood of patients was significantly lower than that in the control group（P〈 0.0001）,and the amount of CD3 ＋ lymphocytes in the patients whose PRL level was less than 100 ng/ml was not different from omit that in the patients whose PRL level was exceeded 100 ng/ml（P〉0.05） .The amount of CD3 ＋ CD4 ＋ lymphocytes in the peripheral blood of patients was not significant different（P〉 0.05）,and the amount of CD3 ＋ CD4 ＋ lymphocytes in the patients whose PRL level was less than 100 ng/ml was no different from the persons that in the patients whose PRL level was exceeded 100 ng/ml（P〈 0.05） .There was no sig-nificant difference about the amount of CD3 ＋ CD8 ＋ lymphocytes in the peripheral blood of patients（P 〉0.05）,and there was no significant difference between the groups which grouped the patients into the PRL levels.The amount of CD4 ＋ CD25 ＋ lymphocytes in the peripheral blood of patients was significantly higher than that in the normal group（P 〈0.0001）,and there was no significant difference between the groups which grouped the patients into the PRL levels.Conclusion It can be inferred that the reason of the reduction of CD3 ＋ T lymphocytes in the peripheral blood of patients with pituitary adenoma is that the increasing of CD4 ＋ CD25 ＋ T lymphocytes resulted the reduction of IL-2,thereby inhibiting T lymphoc

关 键 词：泌乳素 T淋巴细胞 垂体腺瘤 

分 类 号：R736.4[医药卫生—肿瘤]