DNA修复基因多态性与胃癌易感性的关系  

Association of DNA repair gene polymorphisms with genetic susceptibility to gastric cancer

作　　者：邓少丽[1] 陈鸣[1] 陈伟[1] 鲁卫平[1] 黄恒柳[1] 夏季[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所检验科,重庆400042

出　　处：《分子诊断与治疗杂志》2010年第6期371-374,共4页Journal of Molecular Diagnostics and Therapy

基　　金：第三军医大学临床科研基金(2008-1087)

摘　　要：目的分析中国西南地区人群DNA修复基因XRCCl和XPD基因多态性分布，探讨XRCCl和XPD基因多态性与胃癌发病风险的关系。方法采用1：1病例对照研究方法，采用基因测序法检测160例胃癌患者及160例正常人外周血GSTPl基因XPDAsp312Asn，XRCClArgl94Trp，和XRCClArg280His多态性，进行胃癌风险分析。结果胃癌组与正常对照组相比，XPD312、XRCCl280位点的多态性分布频率无明显差异，而XRCCl194Trp的分布频率在病例组和对照组中分别为17．2％和7．3％，具有统计学差异。XRCCl194Trp等位基因的个体其胃癌风险增高（OR=2．72，95％CI=1．04~7．24，P=0．027）。结论XRCClArgl94Trp基因多态性可增加人群患胃癌的风险，XPDAsp312Asn、XRCClArg280His基因多态性与胃癌易感性无关联。Objective To evaluate the single nucleotide polymorphisms （SNPs） of xeroderma pigmentosum group D （XPD） and X-ray repair cross complement 1 （XRCC1） genes in gastric cancer and control subjects in a population from Southwestern China for their association with susceptibility of gastric cancer risk. Methods 160 hospital-based cases and matched controls were recruited and blood samples were collected from each of them and amplified with a PCR and DNA-sequenced for XPD Asp312Asn, XRCC1 Arg194Trp, andXRCC1 Arg280His genotyping. Results Allelic association analysis of these three SNPs showed that the frequency ofXRCC1 194Trp in gastric cancer case and the control was 17.2% and 7.3%, respectively, which was significantly associated with gastric cancer risk （OR=2.72, 95% CI： 1.04-7.24, P=0.027）. However, other SNPs didn＇t show an association with gastric cancer risk or other clinicopathological data of the patients. Conclusion Polymorphism ofXRCC1 Arg194Trp significantly increased the risk of gastric cancer. Future study will verify these findings for use of this SNP as biomarker in gastric cancer.

关 键 词：DNA修复基因 基因多态性 胃癌 XPD XRCC1 

分 类 号：R735.2[医药卫生—肿瘤]