TNF-α在吸烟大鼠脑组织的表达及意义  

Expression of TNF-α in Smoked Rat Brain

作　　者：刘淑杰[1,2] 柳忠兰[3] 宋一晨[4] 刘菲[5] 

机构地区：[1]沈阳二四二医院 [2]沈阳医学院附属第三医院第二门诊部,辽宁沈阳110034 [3]中国医科大学附属第一医院神经内科 [4]中国医科大学 [5]沈阳医学院沈洲医院神经内科

出　　处：《沈阳医学院学报》2010年第1期7-8,21,共3页Journal of Shenyang Medical College

摘　　要：目的:观察不同吸烟量大鼠脑组织TNF-α的表达,初步探讨吸烟导致脑缺血的发病机制。方法:建立吸烟动物模型,将60只健康Wistar大鼠随机分为A组(正常对照组)、B组(非吸烟对照组)、C组(短期吸烟组)、D组(长期小量吸烟组)、E组(长期大量吸烟组)、F组(戒烟组),应用免疫组织化学方法观察各组大鼠脑组织TNF-α的表达。结果:TNF-α阳性细胞呈棕色,A组及B组大鼠脑组织中TNF-α阳性细胞表达极少,两组比异差较无显著性(P>0.05),C-E各组表达比其它各组明显增多,F组TNF-α表达下降,与其它各吸烟组相比差异有显著性(P<0.05)。结论:吸烟导致大鼠脑组织TNF-α的表达升高,戒烟后表达下降,TNF-α表达升高可能是吸烟导致脑梗死的主要途径之一。Objective：To observe the expression of TNF-α on smoked rats brain tissue of different groups,and try to explore the possible mechanisms of TNF-α in ischemic cerebral vascular diseases（ICVD）.Methods：Animal model of smoked rats were made,Method-Healthy Wistar rats were randomly divided into six groups of ten animals for each one.A group（the normal control group）,B group（the none-smoking control group）,C group（short-time smoking group）,D group（long-time and small-dose smoking group）,E group（long-time and big-dose smoking group）,F group（giving-up smoking group）.The immunohistochemical method was used to observe the expression of TNF-α in every group.Results：The positive cells of TNF-α showed brown and located neurocyte neurogliocyte and vascular endothelial cell.There were a few expression in group A and group B（P0.05）.The expression in group C,D,E were stronger than group A,B.The expression in group F was lower than group C,group D and group E（P0.05）.Conclusion：Exposure to smoking can elevate the expression of TNF-α.While giving-up smoking can reduce the expression of TNF-α.The elevating expression of TNF-α may be one of the ways for ICVD after exposured to cigarettes.Giving-up smoking can prevent and reduce the ICVD.Anti-TNF-α maybe a new way for prevention ICVD.

关 键 词：吸烟 大鼠 脑组织 TNF-Α 

分 类 号：R743[医药卫生—神经病学与精神病学]