槐定碱对LPS诱导的RAW264.7巨噬细胞p38、iNOS表达的影响  

Effect of sophoridine on lipopolysaccharide-induced expression of iNOS and p38 in RAW264.7 cells

作　　者：张巍[1] 李业成[2] 黄菱[1] 周娅[1] 

机构地区：[1]宁夏医科大学病原生物学与免疫学系,宁夏银川750004 [2]兰州大学第一医院骨科,甘肃兰州730000

出　　处：《中国药理学通报》2010年第11期1485-1489,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No30660227);宁夏自然科学基金资助项目(NoNZ0783);教育部新世纪优秀人才支持计划(NoHCET-06-0916)

摘　　要：目的探讨槐定碱(sophoridine,SRI)在内毒素导致的炎症反应中的作用。方法采用LPS诱导的RAW264.7巨噬细胞建立细胞炎症反应模型,实验细胞分为5组(n=6):空白对照组、LPS组、槐定碱组、SB203580组、SB203580+槐定碱组,利用反转录聚合酶链反应(RT-PCR)技术检测RAW264.7巨噬细胞p38 mRNA表达量;利用Western blot技术检测RAW264.7巨噬细胞p-p38与iNOS蛋白的表达量。结果槐定碱组与LPS组比较p-p38蛋白表达量和p38 mRNA表达量降低,但高于空白对照组(P<0.01),表明槐定碱可以抑制LPS诱导的RAW264.7巨噬细胞p-p38蛋白表达和p38 mRNA表达量;与LPS组比较,槐定碱组iNOS蛋白表达量降低,但高于空白对照组(P<0.01),SB203580+槐定碱组iNOS蛋白表达量低于槐定碱组和SB203580组(P<0.01),表明槐定碱可以抑制LPS诱导的RAW264.7巨噬细胞P38MAPK信号通路下游iNOS蛋白表达,并且与SB203580有协同作用。结论槐定碱可以通过抑制p38MAPK位点而下调p-p38、iNOS蛋白表达而发挥抗炎作用。Aim To investigate the role of sophoridine （SRI） in inflammatory induced by LPS.Methods In this study,lipopolysaccharide （LPS）-activated macrophage cells（RAW 264.7） was employed as an inflammation model.RAW264.7 cells were divided into 5 groups（n=6）：control group,LPS group,SRI（sophoridine） group,SB203580 group,SB203580＋ SRI group.After activation,expression of p38 mRNA and expression of iNOS protein and p-p38 protein were respectively measured by reverse transcription polymerase chain reaction （RT-PCR） and western blotting.Results Compared with LPS group,expression of p-p38 protein and p38 mRNA were significantly decreased in SRI group （P0.01）,which indicated that sophoridine （SRI） can inhibit expression of p-p38 protein and p38 mRNA induced by LPS in RAW 264.7 cells;Compared with LPS group,the expression of iNOS protein was significantly decreased in SRI group （P0.01）.Compared with SRI（sophoridine） and SB203580 group,the expression of iNOS protein was significantly decreased in SB203580＋SRI（sophoridine） group（P0.01）,which indicated that sophoridine（SRI） can abolish the expression of iNOS protein and be synergistic effect with SB203580 in p38 MAPK pathway.Conclusion sophoridine（SRI） can abolish inflammation by decreasing the expression of iNOS protein and p-p38 protein in p38 MAPK pathway.

关 键 词：槐定碱 脂多糖 SB203580 磷酸化P38 诱导型一氧化氮合酶 RAW264.7巨噬细胞 

分 类 号：R-332[医药卫生] R284.1