肝损伤组织萃取物对人肝癌细胞株BEL-7402诱导分化作用初探  

Differentiation induction of human hepatocellular carcinoma BEL-7402 cells with tissue extracts of injured liver

作　　者：成娜[1,2] 刘大伟[1,2] 刘芳[1,2] 向锦[3] 杨迪[1,2] 赵国强[1,2] 

机构地区：[1]中山大学附属第一医院病理科,广东广州510080 [2]中山大学中山医学院病理学教研室,广东广州510080 [3]中山大学肿瘤防治中心,广东广州510060

出　　处：《中国病理生理杂志》2010年第11期2101-2105,共5页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30170473);广东省自然科学基金资助项目(No.04009326)

摘　　要：目的:观察肝损伤组织萃取物对人肝癌细胞株BEL-7402的影响,探讨肿瘤诱导分化治疗的新途径。方法:以肝损伤模型动物的肝组织萃取物为诱导剂对人肝癌细胞株BEL-7402进行诱导分化,动态观察肝癌细胞形态特点、MTT生长曲线以及细胞周期分布等生物学特征的变化规律。结果:在肝损伤组织萃取物的影响下,肝癌细胞增殖及生长速度减慢,G0/G1期细胞数增加,S期细胞数下降,增殖指数(PI)降低。结论:肝损伤组织萃取物可以影响肝癌细胞BEL-7402的分化状态,促进细胞分化,降低肿瘤特性,显示出作为肿瘤诱导分化剂的重要潜质,值得进一步研究和探讨。AIM： To observe the effects of tissue extracts of injured liver on BEL -7402 cells,and explore a novel strategy of tumor therapy by differentiation induction. METHODS： Differentiation induction of human hcpatocellular carcinoma cell line BEL - 7402 was carried out with liver tissue extracts from an animal model of liver injury. The changes of cell biological characteristics, such as morphological features of the cells, MTI＇ growth curves and cell cycle distribution, were dynamically observed. RESULTS： After exposed to the tissue extracts of injured liver, the number of mitotic cells was decreased, and the speed of growth and the proliferation of carcinoma cells were slowed down dramatically. The per- centage of the cells in G0/GI phase was increased, while the cells in S phase was decreased. The level of proliferation in- dex （PI） also declined. CONCLUSION： The tissue extracts of injured liver affect the differentiation status of human hep- atocellular carcinoma cell line BEL -7402, promote the cell differentiation and reduce the tumor characteristics. The tissue extracts of injured liver possess an important potential as a tumor differentiation inducer.

关 键 词：肝损伤组织萃取物 BEL-7402细胞 诱导分化治疗 

分 类 号：R363[医药卫生—病理学]