同种异体脂肪干细胞门脉移植对大鼠纤维化肝脏的影响  

Effect of portal vein transplantation with allogeneic adipose tissue-derived mesenchymal stem cells on liver fibrosis in SD rats

作　　者：王于[1] 连帆[2] 谭国胜[1] 范文哲[1] 向贤宏[1] 陈伟[1] 杨建勇[1] 李家平[1] 

机构地区：[1]中山大学附属第一医院介入放射科,广东广州510080 [2]中山大学附属第一医院风湿内科,广东广州510080

出　　处：《中国病理生理杂志》2010年第11期2197-2201,共5页Chinese Journal of Pathophysiology

基　　金：广东省科技计划资助项目(No.B36003002);广东省自然科学基金资助项目(No.7001663)

摘　　要：目的:研究同种异体脂肪干细胞肝内移植对大鼠纤维化肝脏的影响。方法:从SD大鼠腹膜脂肪组织提取脂肪干细胞,纯化、培养并稳定传代。雄性SD大鼠48只,成功建立大鼠肝纤维化模型。实验分为对照组(n=14)、门静脉组(n=11)和尾静脉组(n=14)。后两组分别经门静脉、尾静脉注射同种异体移植脂肪干细胞,观察移植前后大鼠肝血流动力学变化,并比较门静脉组和尾静脉组的疗效差异。结果:成功提取脂肪干细胞,纯化、培养并稳定传代。输注脂肪干细胞前后各组肝脏计算机断层扫描灌注指数显示,门静脉组肝脏血流灌注有明显改善,且以门静脉灌注量为主(P<0.05),尾静脉组仅有轻微改善。组织学肝硬化评分显示,门静脉组见肝纤维化、肝细胞脂肪变性、肝细胞变性坏死均明显改善(P<0.05);尾静脉组仅肝细胞脂肪变性有改善。免疫组化示门静脉组肝脏微血管密度较其它组明显减少(P<0.05)。结论:同种异体脂肪干细胞移植能改善肝纤维化SD大鼠的肝脏血流灌注及病理学改变;门静脉移植途径较尾静脉途径疗效更显著。AIM： To explore the influence of adipose tissue-derived mesenchymal stem cells （ADMSCs） transplantation on hepatic fibrosis in rats. METHODS： ADMSCs from abdominal lipid tissues were extracted, cultured and passaged. The hepatic fibrosis rat model was built up and randomly divided into 3 groups： hepatic cirrhosis group （n=14）; portal vein transplantation group （n=11） and caudal vein transplantation group （n=14）. Computer tomography（CT） perfusion index, histological scores and microvessel density were detected and compared after transplantation of ADMSCs among the 3 groups. RESULTS： After transplantation of ADMSCs, the total hepatic blood perfusion, especially portal vein perfusion, significantly increased in portal vein transplantation group determined by CT perfusion scan （P〈0.05）, but slightly increased in caudal vein transplantation group. The histological scores showed significant alleviation of fibrosis evidence in portal vein transplantation group, and slightly change of adipose degeneration in caudal vein transplantation group. Microvessel density decreased significantly in portal vein transplantation group as compared to the other 2 groups （P〈0.05）. CONCLUSION： Transplantation of ADMSCs greatly helps the alleviation of hepatic fibrosis. Portal vein transplantation benefits more than caudal vein transplantation.

关 键 词：肝纤维化 脂肪干细胞 微血管密度 肝血流动力学 

分 类 号：R[医药卫生]