白内障晶状体上皮细胞中色素上皮细胞衍生因子的表达  

Expression of the pigment epithelium-derived factor in human cataract lens epithelial cells

作　　者：刘静[1] 刘冰[2] 张红松[1] 金姬[3] 关明[4] 彭智培[5] Dennis Lam 

机构地区：[1]中国北京市中日友好医院眼科,100029 [2]吉林大学第一临床医院血库,中国吉林省长春市130051 [3]中国浙江省金华市中心医院眼科,321075 [4]复旦大学华山医院检验中心,中国上海市201203 [5]香港中文大学眼科及视觉科学系

出　　处：《国际眼科杂志》2010年第12期2253-2255,共3页International Eye Science

摘　　要：目的:观察色素上皮细胞衍生因子(pigment epithelium-derived factor,PEDF)在人类白内障晶状体上皮细胞中的表达,以探讨PEDF在白内障发病机制中的作用。方法:白内障超声乳化手术中,实施前囊膜环形撕囊时摘取晶状体前囊连同晶状体上皮细胞,采用免疫组化的方法检测PEDF和VEGF在晶状体上皮细胞中的表达。结果:老年性白内障晶状体上皮细胞虽然PEDF存在高强度表达,与VEGF相比,这种表达还是较弱。结论:老年性白内障晶状体上皮细胞存在PEDF和VEGF的表达,而且二者之间存在着一定的相互作用,由此推断PEDF和VEGF在老年性白内障的发病机制中处于重要的作用。AIM： To observe the expression of pigment epitheliumderived factor （PEDF） in human cataract lens epithelial cells, and to explore its role in the pathogenesis of cataract. METHODS. During the cataract circular capsulorhexis of phacoemulsification surgery, the anterior lens capsule and lens epithelial cells were obtained and fixed. Immunohistochemical method was used to determine the expression of of PEDF and VEGF in lens epithelial cells. RESULTS： There were expression of PEDF and VEGF in senile cataract crystal epithelial cells, while the high expression of PEDF was weaker than that in the VEGF. CONCLUSION： PEDF and VEGF are expressed in senile cataract lens epithelial cells and both exist certain interactions. It may plays an important role for the pathogenesis of senile cataract.

关 键 词：色素上皮细胞衍生因子 晶状体上皮细胞 老年性白内障 白内障超声乳化 免疫组化 

分 类 号：R776.1[医药卫生—眼科]