大鼠视网膜缺血性再灌注中HIF-1α与SDF-1的表达意义  

Expression and significance of HIF-1α and SDF-1 in retinal ischemia-reperfusion in rats

作　　者：曲来强[1] 张文芳[1] 鲁建华[1] 刘瑜[1] 

机构地区：[1]兰州大学第二医院眼科,中国甘肃省兰州市730030

出　　处：《国际眼科杂志》2010年第12期2265-2267,共3页International Eye Science

摘　　要：目的:探讨缺氧诱导因子-1α(hypoxia-inducible factor-1α,HIF-1α)和基质细胞衍生因子-1(stromal cell-derived factor-1,SDF-1)在缺血性视网膜疾病及视网膜缺血再灌注(retinal ischemia-reperfusion,RIR)发展中的表达规律及二者的相互关系。方法:应用免疫组织化学的方法分析HIF-1α和SDF-1在视网膜缺血再灌注模型中的表达及其关系。结果:在对照组中HIF-1α和SDF-1未见表达或轻微表达,视网膜缺血性再灌注组0h开始表达,6h表达增强,12h表达最强,24h表达减弱,以后随着时间推移表达逐渐下降。结论:HIF-1α和SDF-1表达与视网膜缺血再灌注中视网膜功能损伤密切相关,同时二者具有协同相关性。AIM： To investigate the expression pattern and correlation of hypoxia-inducible factor-1α（HIF-1α） and stromal cell-derived factor-1（SDF-1） in the ischemic retinopathy and retinal ischemia-reperfusion. METHODS： In order to analyze the correlation and expression between HIF-1α and SDF-1 in retinal ischemia-reperfusion model, immunohistochemical method was used. RESULTS： In the control group, HIF-1α and SDF-1 were not expressed or slightly expressed, while that in retinal ischemia-reperfusion group began to express immediately after carotid artery ligation, ascended at 6 hour, reached peak at 12 hour, and decreased gradually after 24 hours. CONCLUSION：HIF-1α and SDF-1 expression are closely relate to retinal ischemia-reperfusion injury, and have a collaborative relationship.

关 键 词：视网膜 缺血再灌注 低氧 缺氧诱导因子-1Α 基质细胞衍生因子-1 相关性 

分 类 号：R774.1[医药卫生—眼科]