TGF-β1体外对人眼眶成纤维细胞3H-Pro掺入的影响  

Influence of TGF-β1 on human orbital fibroblasts by 3H-Pro absorption in vitro

作　　者：高自清[1] 方丽[1] 周琦[1] 李娟[1] 岳晓丽[1] 李宁[1] 

机构地区：[1]蚌埠医学院第一附属医院眼科,中国安徽省蚌埠市233004

出　　处：《国际眼科杂志》2010年第12期2271-2272,共2页International Eye Science

摘　　要：目的:观察转化生长因子β1(transforming growth factor-β1,TGF-β1)对人眼眶成纤维细胞(orbital fibroblast,OF)3H-Pro掺入的影响。方法:体外培养人OF,用0.1,1.0,5.0,10.0,100.0μg/LTGF-β1对细胞进行处理,用3H-Pro掺入法测定细胞放射性强度来判断细胞胶原合成。结果:用0.1,1.0,5.0,10.0μg/L TGF-β1作用后OF3H-Pro掺入量增加。100.0μg/L TGF-β1对OF作用相反。结论:TGF-β对人OF合成胶原有双相调节性。AIM： To investigate the influence of transforming growth factor-β1（TGF-β1） on human orbital fibroblast（OF） by 3H-Pro absorption in vitro. METHODS： Human OF were cultured in vitro. Different concentrations of TGF-β1（0.1,1.0,5.0,100,100.0μg/L） were incubated with cultured human OF. The collagen synthesis was evaluated by 3H-Pro absorption. RESULTS： TGF-β1 of 0.1, 1.0,5.0,10.0μg/L could promote the 3H-Pro absorption more than the control group while the concentration of TGF-β1 of 100.0μg/L had a different result. CONCLUSION： There is a concentration-dependant effect between TGF-β1 and collagen synthesis of OF.

关 键 词：眼眶成纤维细胞 转化生长因子Β1 胶原合成 增生性玻璃体视网膜病变 

分 类 号：R774.1[医药卫生—眼科]