Increased stromal-cell-derived factor 1 enhances the homing of bone marrow derived mesenchymal stem cells in dilated cardiomyopathy in rats  

作　　者：ZHOU Yan-li ZHANG Hai-feng LI Xin-li DI Ruo-min YAO Wen-ming LI Dian-fu FENG Jian-lin HUANG Jun CAO Ke-jiang Michael Fu 

机构地区：[1]Department of Cardiology , First Affiliated Hospital of Nanjing Medical University/Jiangsu Province Hospital, Nanjing, Jiangsu 210029, China [2]Department of Nuclear Medicine , First Affiliated Hospital of Nanjing Medical University/Jiangsu Province Hospital, Nanjing, Jiangsu 210029, China [3]Depatrtment of Medicine/Wallenberg Laboratory, Sahlgrenska University Hospital, Gothenburg, Sweden

出　　处：《Chinese Medical Journal》2010年第22期3282-3287,共6页中华医学杂志（英文版）

基　　金：This study was supported by the National Natural Science Foundation of China （No. 30900603）, Specialized Research Fund for the Doctoral Program of Higher Education （No. 20093234110008 and No. 20093234120008）, and Project Supported by Department of Health of Jiangsu Province （No. ZX07 200902）

摘　　要：Background Stem cell transplantation has been shown to have beneficial effects on dilated cardiomyopathy. However, mechanism for stem cell homing to cardiac tissue in dilated cardiomyopathy has not yet been elucidated. Methods Mesenchymal stem cells were obtained from rat bone marrow, expanded in vitro, and labeled with 99mmc. Cardiomyopathy model was induced by doxorubicin in rats. 9ernTC labeled cells were infused into the left ventricles in cardiomyopathy and control rats. Sixteen hours after injection, animals were sacrificed and different tissues were harvested to measure specific radioactivity. By use of real-time polymerase chain reaction and immunohistochemistry, mRNA and protein expressions for stromal-cell-derived factor 1 in cardiac tissue were measured. Results Labeling efficiency of mesenchymal stem cells was （70.0±11.2）%. Sixteen hours after mesenchymal stem cell transplantation, the heart-to-muscle radioactivity ratio was increased significantly in cardiomyopathy hearts as compared to control hearts. Both mRNA and protein expressions of cardiomyopathy hearts as compared with control hearts. Conclusion In dilated cardiomyopathy induced by doxorubicin in heart may induce mesenchymal stem cells home to the heart. stromal-cell-derived factor 1 were up-regulated in up-regulated expression of stromal-cell-derived factor IBackground Stem cell transplantation has been shown to have beneficial effects on dilated cardiomyopathy. However, mechanism for stem cell homing to cardiac tissue in dilated cardiomyopathy has not yet been elucidated. Methods Mesenchymal stem cells were obtained from rat bone marrow, expanded in vitro, and labeled with 99mmc. Cardiomyopathy model was induced by doxorubicin in rats. 9ernTC labeled cells were infused into the left ventricles in cardiomyopathy and control rats. Sixteen hours after injection, animals were sacrificed and different tissues were harvested to measure specific radioactivity. By use of real-time polymerase chain reaction and immunohistochemistry, mRNA and protein expressions for stromal-cell-derived factor 1 in cardiac tissue were measured. Results Labeling efficiency of mesenchymal stem cells was （70.0±11.2）%. Sixteen hours after mesenchymal stem cell transplantation, the heart-to-muscle radioactivity ratio was increased significantly in cardiomyopathy hearts as compared to control hearts. Both mRNA and protein expressions of cardiomyopathy hearts as compared with control hearts. Conclusion In dilated cardiomyopathy induced by doxorubicin in heart may induce mesenchymal stem cells home to the heart. stromal-cell-derived factor 1 were up-regulated in up-regulated expression of stromal-cell-derived factor I

关 键 词：mesenchymal stem cells stromal-cell-derived factor 1 CARDIOMYOPATHY 

分 类 号：Q987[生物学—遗传学] Q254[生物学—人类学]