p42/44MAPK抑制剂U0126对人胃癌SGC-7901细胞增殖和凋亡的影响  

Mechanism of Effect of p42/44MAPK Inhibitor U0126 on SGC-7901 Cell Proliferation and Apoptosis

作　　者：李斌[1] 陈鹏[2] 

机构地区：[1]西安医学院附属医院肿瘤科,西安710077 [2]西安医学院生物化学教研室

出　　处：《肿瘤防治研究》2010年第11期1234-1237,共4页Cancer Research on Prevention and Treatment

摘　　要：目的研究U0126对人胃癌SGC-7901细胞生物学特性的影响。方法用MTT法测定U0126对SGC-7901细胞增殖的影响,流式细胞仪检测U0126对SGC-7901细胞周期和细胞凋亡的影响,用Western blot分析p42/44和p38以及其磷酸化水平的变化。结果 10、15、20μMU0126均能够抑制胃癌SGC-7901细胞的增殖;可使S和G2/M期肿瘤细胞比例减少,G0/G1期肿瘤细胞比例增加,其中20μM浓度的U0126作用最强(P<0.01);U0126诱导肿瘤细胞凋亡,其中20μM浓度的U0126凋亡作用最强(P<0.01);U0126使p-p42/44下调而使p-p38上调。结论 U0126诱导肿瘤细胞凋亡,抑制肿瘤细胞的增殖,其机制可能是抑制p-p42/44表达,并使p-p38表达增强。Objective To study the effect of U0126 on cytobiological characteristics of SGC-7901.Methods To determine effect of U0126 on SGC-7901 cell proliferation by MTT.Flow cytometry was used to detect influence of U0126on SGC-7901 cell cycle and apoptosis.The change of p42/44,p38,p-p42/44 and p-p38 was analyzed by Western blot.Results 10,15,20μM U0126 can restrain SGC-7901 cell proliferation,decrease S and G2/M phase cell ratio and increase G0/G1 phase cell ratio.U0126 can induce tumor cell apoptosis and have a most powerful apoptosis in 20μM（P〈0.01）.U0126 can decrease p-p42/44 and increase p-p38.Conclusion U0126 can induce tumor cell apoptosis and inhibit cell proliferation.

关 键 词：增殖 周期 凋亡 p42/44MAPK U0126 胃癌 

分 类 号：R73-36[医药卫生—肿瘤] R735.2[医药卫生—临床医学]