人次级淋巴组织趋化因子对宫颈癌Siha细胞侵袭能力的影响  

Effect of Human Secondary Lymphoid Tissue Chemokine on Invasive Ability of Cervical Cancer Cell Line Siha

作　　者：刘宏杰[1] 胡萍[2] 张志丽[3] 卢晓[3] 沈关心[4] 吴砂[3] 

机构地区：[1]广州市第一人民医院胃肠外科,广州510510 [2]暨南大学医学院免疫学教研室 [3]南方医科大学基础医学院免疫学教研室 [4]华中科技大学同济医学院免疫学系

出　　处：《肿瘤防治研究》2010年第11期1238-1240,共3页Cancer Research on Prevention and Treatment

基　　金：广东省科技攻关项目(2008B030301001);广东省自然科学基金博士启动项目(K1021187);南方医科大学基础医学院院长基金项目(B1100363)

摘　　要：目的研究人次级淋巴组织趋化因子(SLC)对Siha细胞侵袭能力的影响。方法从人脾脏cDNA扩增出SLC,构建真核表达载体,转染宫颈癌细胞株Siha。Transwell检测肿瘤细胞侵袭能力,利用NF-κB特异性启动子荧光素酶报告系统检测肿瘤细胞内NF-κB的活化。结果经PCR、酶切、测序及ELISA证实,SLC真核表达载体构建表达成功。SLC转染Siha细胞,肿瘤侵袭能力增强约(2.34±0.14)倍,且肿瘤细胞内NF-κB的活化增强约(3.98±0.194)倍。结论 SLC作为趋化因子,其作用已不局限于趋化作用,还可诱导肿瘤细胞NF-κB的活化,促进肿瘤细胞侵袭,有着多重作用。Objective To investigate the effect of human secondary lymphoid tissue chemokine（SLC） on invasive ability ability of Siha cell line.Methods The SLC gene was amplified from human spleen cDNA,and constructed into eukaryotic expression vector pDsRed2-N1.The plasmid was transfected into human cervical cancer Siha cell line.The tumor invasive ability was detected using transwell,and the activation of NF-κB was tested by NF-κB specific promoter Luciferase reporter system.Results The SLC plasmid was constructed and transfected Siha cell successfully,with PCR,enzyme digestion and ELISA.Siha-CCL21 had obviously increased ability of tumor invasion（2.34±0.14 times）and NF-κB activation（3.98±0.194 times）compared with controls.Conclusion Although as a chemoattractant,the chemokine SLC plays multiple roles,including promotion of NF-κB activation and invasion of cancer cells.

关 键 词：人次级淋巴组织趋化因子SLC 宫颈癌 侵袭 NF-ΚB 

分 类 号：R73-35[医药卫生—肿瘤] R392.1[医药卫生—临床医学]