胃肠富集、肠道富集Krüppel样因子在人胃癌组织和细胞株中的表达  

Expression of GKLF and IKLF in Human Gastric Carcinoma Tissues and Cell Lines

作　　者：严辉[1] 陈卫昌[1] 岑建农 沈宏杰 顾冬梅[3] 郭凌川[3] 

机构地区：[1]苏州大学附属第一医院消化内科,江苏苏州215006 [2]江苏省血液研究所 [3]苏州大学附属第一医院病理科,江苏苏州215006

出　　处：《肿瘤防治研究》2010年第11期1269-1272,共4页Cancer Research on Prevention and Treatment

基　　金：江苏省135医学重点人才基金资助项目(RC2007076)

摘　　要：目的研究96例胃癌组织和胃癌细胞株(MKN-28、SGC-7901、BGC-823、HGC-27)中胃肠富集Krüppel样因子(Gut-enriched Krüppel-like factor,GKLF)、肠道富集Krüppel样因子(Intestinal-enriched Krüppel-like factor,IKLF)的表达及意义。方法荧光实时定量PCR法检测胃癌组织和胃癌细胞株中GKLFmRNA及IKLF mRNA的水平;分别采用免疫组织化学法和Western blot检测胃癌组织和细胞株中GKLF、IKLF蛋白的表达。结果胃癌组织中GKLF mRNA水平低于正常胃黏膜,IKLF mRNA水平高于正常胃黏膜(P均<0.0001),GKLF mRNA、IKLF mRNA的水平与胃癌分化程度无关。胃癌组织中GKLF蛋白阳性率显著低于正常胃黏膜,IKLF蛋白阳性率则显著高于正常胃黏膜(P均=0.001);GKLF、IKLF蛋白表达与胃癌患者的年龄、性别、分化程度、Lauren分型无关,但与淋巴结是否有转移及pTNM分期相关(P=0.001)。胃癌细胞株中GKLF mRNA及蛋白水平低于人正常胃上皮细胞株GES-1,IKLF mRNA水平及蛋白则高于细胞株GES-1(P均<0.05)。结论 GKLF、IKLF可能参与了胃癌的发生、发展,检测GKLF、IKLF的表达有助于判断胃癌患者病情程度和预后。Objective To study the expression and clinical significance of GKLF and IKLF in 96 human gastric carcinoma tissues and cell lines（MKN-28,SGC-7901,BGC-823,HGC-27）.Methods The mRNA levels of GKLF and IKLF in gastric carcinoma tissues and cell lines were determined by real-time fluorescence quantitative PCR.The protein levels of GKLF and IKLF in gastric carcinoma tissues and cell lines were detected by immunohistochemistry and Western blot,respectively.Results The GKLF mRNA level in gastric carcinoma tissues was lower than that in normal gastric mucosa while IKLF mRNA level was higher in the same condition（P〈0.0001）.Furthermore,the mRNA levels of GKLF and IKLF in gastric carcinoma tissues were uncorrelated with differentiation.Positive expression rate of GKLF protein in the carcinoma tissues was significantly lower than that in normal gastric mucosa while positive expression rate of IKLF protein was significantly higher（P=0.001）.The expressions of GKLF and IKLF protein were uncorrelated with age,gender,differentiation and Lauren＇s classification but correlated with lymph node involvement and clinical stage（P=0.001）.The expressions of GKLF mRNA and protein in gastric carcinoma cell lines were lower than that in GES-1while the expressions of IKLF mRNA and protein were higher（P〈0.05）.Conclusion GKLF and IKLF may play roles in the carcinogenesis and progression of gastric carcinoma.To examine the expression of GKLF and IKLF may be useful in diagnose the degree and prognosis of gastric carcinoma.

关 键 词：胃癌 胃肠富集Krüppel样因子 肠道富集Krüppel样因子 

分 类 号：R735.2[医药卫生—肿瘤]