Seven amino acid peptide mimic from HVR1 of HCV influences cytokine profile of immune cells  

作　　者：Song Chunhui Chen Liming Yang Bin Chi Shuping Cheng Yun 

机构地区：[1]Department of Liver Disease Research Center for Biology, No. 302 Hospital of PLA ,Beijing 100039, China

出　　处：《Journal of Medical Colleges of PLA(China)》2010年第5期257-266,共10页中国人民解放军军医大学学报（英文版）

基　　金：Supported by the National High Technology Research and Development Program of China(863 Program,No. 2006AA022494)

摘　　要：Objective:To investigate the actions of cytokine profile in the immune cells by a seven amino acid peptide mimic from HVR1 of HCV (GQTYTSG,named 7P).Methods:The peripheral blood mononuclear cells (PBMCs) from 17 healthy blood donors were stimulated with 7P,and the cytokine levels in CD4+CD8-,CD4-CD8+ T cells,NK,NKT cells were analyzed by the intracellular cytokine staining.Results:The frequency of cells which secreted interferon-γ (IFN-γ) was found to be significantly increased in all cells,interleukin-10(IL-10) was significantly increased in CD4+CD8-,CD4-CD8+ T cells but decreased in NK,NKT cells,and tumor necrosis factor-α (TNF-α) was decreased in CD4+CD8-,CD4-CD8+ but increased in NK cells.Conclusion:7P could induce a cytokine profile in different immune cells in vitro and there was some difference between the CD4+CD8-,CD4-CD8+ T cells which represented the adaptive immunity cells and NK,NKT cells which represented the innate immunity cells.This kind of variation of cytokine profile might contribute to anti-virus and anti-inflammatory immune reaction.Objective： To investigate the actions of cytokine profile in the immune cells by a seven amino acid peptide mimic from HVR1 of HCV （GQTYTSG, named 7P）. Methods： The peripheral blood mononuclear cells （PBMCs） from 17 healthy blood donors were stimulated with 7P, and the cytokine levels in CD4＋CD8-,CD4-CD8＋ T cells, NK, NKT cells were analyzed by the intracellular cytokine staining. Results： The frequency of cells which secreted interferon-γ （IFN-γ） was found to be significantly increased in all cells, interleukin-10（IL-10） was significantly increased in CD4＋CD8 , CD4 CD8＋ T cells but decreased in NK, NKT cells, and tumor necrosis factor-α （TNF-α） was decreased in CD4＋CD8- , CD4 CD8＋ but increased in NK cells. Conclusion： 7P could induce a cytokine profile in different immune cells in vitro and there was some difference between the CD4＋CD8- ,CD4 CD8＋ T cells which represented the adaptive immunity cells and NK, NKT cells which represented the innate immunity cells.This kind of variation of cytokine profile might contribute to anti-virus and anti-inflammatory immune reaction.

关 键 词：Seven amino acids peptide mimic Interleukin-lO INTERFERON-Γ Tumor necrosis factor-α Hepatitis C virus 

分 类 号：Q78[生物学—分子生物学] TS201.6[轻工技术与工程—食品科学]