双功能抗体介导CIK细胞杀伤胃癌细胞的实验研究  

The cytotoxicity activity of CIK cells against gastric cancer cell lines mediated by bispecific antibody

作　　者：张林[1] 侯艳红[2] 段云卉[1] 李丹[1] 

机构地区：[1]解放军第309医院消化科,北京100091 [2]解放军第309医院门诊部,北京100091

出　　处：《胃肠病学和肝病学杂志》2010年第11期978-981,共4页Chinese Journal of Gastroenterology and Hepatology

摘　　要：目的初步探讨抗EGFR/抗CD3双功能抗体介导对于CIK细胞杀伤胃癌细胞MKN45及SGC7901能力的影响。方法通过化学耦联法合成抗EGFR/抗CD3双功能抗体并检验其双特异抗体功能。采用免疫细胞化学法明确靶细胞的EGFR表达情况。通过细胞结合率检测及MTT杀伤实验检测抗EGFR/抗CD3双功能抗体介导CIK细胞与胃癌细胞结合及杀伤的能力,并与单纯CIK细胞、单纯EGFR单抗和/或CD3单抗介导CIK细胞的结合及杀伤作用比较。结果抗EGFR/抗CD3双功能抗体介导CIK细胞与胃癌细胞株MKN45及SGC7901结合率分别为(64.6±7.2)%和(59.5±6.1)%,显著高于其余各对照组(P<0.05),对照组间无显著性差异;MTT细胞杀伤实验结果提示:抗EGFR/抗CD3双功能抗体介导CIK细胞对胃癌细胞株杀伤率显著高于单纯抗CD3单抗介导及抗CD3单抗联合抗EGFR单抗介导组,而后两组杀伤率又显著高于单纯抗EGFR单抗介导组及单纯CIK细胞组(P<0.05)。结论体外实验初步显示抗EGFR/抗CD3双功能抗体介导可显著提高CIK细胞的对胃癌细胞的结合及杀伤能力。Objective To investigate the cytotoxicity activity of CIK cells targeted by EGFR/CD3 bispecific antibody （ BsAb） against gastric cancer line SGC7901 and MKN45. Methods A bispecific antibody was constructed by chemically crosslinking one monoclone antibody against human CD3 and one monoclone antibody （ McAb） against human EGFR. The immunocytochemistry assay was performed to detect the expression of EGFR in SGC7901 and MKN45 cell lines. The cytotoxicity activity of CIK cells targeted by EGFR/CD3 BsAb was analyzed by the cell combination rate assay and MTT assay,then the cytotoxicity activity of CIK cells targeted by EGFR/CD3 BsAb,CIK cells targeted by EGFR McAb or/and CD3 McAb and CIK cells were compared. Results The combination rates of CIK cells targeted by EGFR/CD3 BsAb with MKN45 cell and SGC7901 cell were （ 64. 6 ± 7. 2） % and （ 59. 5 ± 6. 1） % respectively,which were significantly higher than those of control groups （ P 0. 05） . The cell lysis rate of CIK cells targeted by EGFR/ CD3 BsAb was higher than that of CIK cells targeted by CD3 McAb only and CIK cells targeted by CD3 McAb and EGFR McAb. The lysis rates of the latter two groups were significantly higher than those of CIK cells targeted by EGER McAb only and CIK cells （ P 0. 05） . Conclusion It is suggested that EGFR/CD3 BsAb could enhance the ability of CIK cells to bind and kill the gastric cancer cells

关 键 词：胃癌 CIK细胞 双功能抗体 

分 类 号：R735.2[医药卫生—肿瘤]