MMP-10在人膀胱癌T24细胞系体外侵袭中的作用  

The role of matrix metalloproteinase-10(MMP-10) on the invasion of human bladder cancer T24 cell line in vitro

作　　者：屈卫星[1] 惠荣[1] 陈伟[2] 丁上书[2] 程永毅[1] 贺大林[2] 

机构地区：[1]西安交通大学医学院第三附属医院泌尿外科,陕西西安710068 [2]西安交通大学医学院第一附属医院泌尿外科,陕西西安710061

出　　处：《现代肿瘤医学》2010年第12期2305-2307,共3页Journal of Modern Oncology

基　　金：陕西省自然科学基础研究计划项目(编号:SJ08-ZT09)

摘　　要：目的:探讨基质金属蛋白酶MMP-10在人膀胱癌细胞体外侵袭中的作用。方法:设计并合成针对MMP-10的siRNA和阴性对照siRNA,以脂质体转染法将其分别导入具有体外高侵袭潜能的T24细胞系,RT-PCR和Western blot法检测MMP-10基因及蛋白表达水平。Transwell体外侵袭实验比较干扰MMP-10前后T24细胞体外侵袭潜能的变化。结果:MMP-10-siRNA可有效沉默MMP-10基因的表达,并降低T24细胞系的体外侵袭能力。结论:MMP-10可能在膀胱癌侵袭转移过程中发挥重要作用,抑制MMP-10可为预防及治疗转移性膀胱癌提供新的思路。Objective：To investigate the role of matrix metalloproteinase-10（MMP-10） on the invasion of human bladder cancer in vitro.Methods：siRNA against MMP-10 gene and negative control siRNA were synthesized,and transfected into human bladder cancer T24 cell line,which has high invasive potential in vitro,.The expression of MMP-10 was detected by RT-PCR and Western blot,while the invasive potential in vitro was also compared by transwell assay.Results：siRNA against MMP-10 gene effectively inhibited the expression of MMP-10 as well as the in vitro invasive potential of bladder cancer T24 cell.Conclusion：MMP-10 may promote the invasion and metastasis in bladder cancer.Inhibition of MMP-10 may provide a new basis for the prevention and treatment of metastatic bladder cancer.

关 键 词：膀胱癌 基质金属蛋白酶10 侵袭转移 RNA干扰 

分 类 号：R730.54[医药卫生—肿瘤] R737.14[医药卫生—临床医学]