Hep-2细胞总RNA转染树突状细胞诱发抗喉癌特异性免疫效应的研究  

Dendritic cells pulsed with Hep-2 cell total RNA induce cytotoxic iymphocytes and elicit antitumor immunity

作　　者：林嘉盈[1] 李晓艳[2] 

机构地区：[1]上海交通大学附属第一人民医院耳鼻咽喉头颈外科,上海200080 [2]上海交通大学附属儿童医院耳鼻咽喉头颈外科,上海200040

出　　处：《现代肿瘤医学》2010年第12期2308-2312,共5页Journal of Modern Oncology

基　　金：上海市科委浦江人才基金项目(编号:07pj14064)

摘　　要：目的:研究转染喉鳞状细胞癌总RNA对树突状细胞(DC)免疫功能的影响,观察肿瘤总RNA修饰后的DC在体外诱导高效而特异的抗喉癌免疫效应。方法:采用分离脐血单核细胞体外诱导DC,Trizol法提取喉鳞状细胞癌细胞株Hep-2总RNA后转染入DC,流式细胞仪分析DC表面分子CD40、CD80、CD83、CD86的表达,LDH法评估转染肿瘤总RNA的DC瘤苗的细胞毒性T淋巴细胞反应。结果:转染后的DC表面分子表达CD40(60.6±0.15)%,CD80(60.2±0.21)%,CD83(85.1±0.06)%,和CD86(91.9%±0.09)%,而在未转染的未成熟DC中低表达;转染RNA的DC瘤苗对于Hep-2细胞株有特异性杀伤效应,在E/T为40:1时,杀伤效率达45.6%,而对对照组靶细胞杀伤效率较低。结论:喉鳞状细胞癌总RNA转染修饰的DC能特异性诱导细胞毒性T淋巴细胞,显著提高DC的抗原提呈功能,在体外能诱导高效而特异的抗喉癌免疫效应。Objective：To investigate the effect of dendritic cells（DCs） transfected with laryngeal squamous cell carcinoma cell line Hep-2 cells total RNA,and to observe the specific anti-laryngeal cancer immunological results induced by modified DC in vitro.Methods：DCs were generated from the cord blood monocytes.Total RNA was isolated from laryngeal squamous cell carcinoma cell line Hep-2 cells by Trizol,and was transfected into DCs.The expression of cell surface molecules CD40,CD80,CD83,and CD86 were detected using flow cytometry.The competence of inducing specific cytotoxic T lymphocyte（CTLs） was assessed with LDH.Results：DC surface molecules CD40（60.6 ±0.15） %,CD80（60.2 ± 0.21） %,CD83（85.1 ± 0.06） %,and CD86（91.9% ± 0.09） % were highly expressed in Hep-2 RNA-DC than that in untreated DC.The cytolytic rate to laryngeal cancer cell was 45.6% at 40：1 E/T ratio.However,no significant cytolytic activity was seen in control cell lines.Conclusion：DCs transfected with Hep-2 cells total RNA could induce specific cytotoxic T lymphocytes,increase the antigen presenting function of DC,and induce specific CTL killing activity to laryngeal squamous cell carcinoma.

关 键 词：树突状细胞 喉鳞状细胞癌 全肿瘤核糖核酸 免疫治疗 

分 类 号：R730.51[医药卫生—肿瘤] R739.65[医药卫生—临床医学]