谷胱甘肽转硫酶P_1基因型与溃疡性结肠炎的相关性  

Correlation Between the Genotypes of Glutathione S-Transferase P_1 and Ulcerative Colitis

作　　者：叶旭军[1] 王桦[1] 袁胜[1] 金雅磊[1] 陈敏[1] 夏冰[1] 

机构地区：[1]武汉大学中南医院综合医疗科,湖北武汉430071

出　　处：《武汉大学学报（医学版）》2010年第6期800-803,共4页Medical Journal of Wuhan University

基　　金：湖北省自然科学基金项目(编号:2008CDB167)

摘　　要：目的:探讨谷胱甘肽转硫酶(GSTs)P1基因型与湖北汉族人群溃疡性结肠炎(UC)的关系。方法:在270例UC患者和623例正常对照中,先采用聚合酶链反应技术(PCR)检测GSTP1基因多态性;然后随机选取65例UC患者和90例性别、年龄相匹配的健康对照者,采用酶联免疫吸附(ELISA)法检测空腹血浆GSTpi水平。结果:与正常对照组相比,GSTP1(VaL/VaL)基因型频率在UC患者中明显增高(48.89%比34.35%,P=0.0004)。进一步根据UC临床症状分层分析,在远端结肠炎患者中GSTP1(VaL/VaL)基因型的分布频率高于广泛结肠炎(57.64%比29.27%,P=0.001);而GSTP1突变基因型与UC患者病情严重程度无关(29.54%比45.46%,P=0.065)。与UC患者相比较,正常对照组中血浆GSTpi水平明显增高[(42.37±22.47)mmol/L比(65.40±51.59)mmol/L,P=0.009]。结论:突变的GSTP1基因型及血浆GSTpi水平与湖北汉族UC明显相关。Objective：To investigate the association between the genotypes of GSTP1and susceptibility to ulcerative colitis（UC）in Chinese Han population.Methods：Two hundred and seventy consecutive patients with ulcerative colitis（UC）and 623age-and sex-matched healthy controls were recruited in the present study,in order to evaluate the prevalence of GSTP1genetic polymorphisms and examine the plasma levels of GSTpi.Results：The frequencies of GSTP1（VaL/VaL） were significantly higher in UC patients than in the controls（48.89%vs 34.35%,P=0.0004）.After stratification of the UC patients according to clinical features,the frequency of GSTP1（VaL/VaL）genotype was also higher in distal colitis than in extensive colitis（57.64% vs 29.27%,P=0.001）.However,the variant genotypes of GSTP1were not significantly linked to severity of disease（29.54%vs 45.46%,P=0.065）.In addition,plasma GSTpi levels were significantly higher in the controls than in UC patients（[65.40±51.59]mmol/Lvs[42.37±22.47] mmol/L,P=0.009）.Conclusion：The GSTP1variant genotypes and plasma phenotype were strongly correlated with UC in Hubei Hans.

关 键 词：谷胱甘肽转硫酶 基因型 溃疡性结肠炎 易感性 

分 类 号：R574.62[医药卫生—消化系统]