不同前列腺组织中雄激素受体的表达研究  

Expressions of the androgen receptor in normal prostate,benigh prostatic hyperplasia and prostate cancer

作　　者：曾锐[1,2] 刘智勇[1] 孙颖浩[1] 许传亮[1] 高旭[1] 矫力[1] 

机构地区：[1]第二军医大学附属长海医院泌尿外科,上海200433 [2]兰州军区乌鲁木齐总医院泌尿外科,新疆乌鲁木齐830000

出　　处：《中华男科学杂志》2010年第11期967-972,共6页National Journal of Andrology

基　　金：国家自然科学基金(30500503)~~

摘　　要：目的:研究雄激素受体(AR)在正常前列腺、良性前列腺增生(BPH)和前列腺癌(PCa)组织中的表达,探讨AR与BPH和PCa的关系。方法:采用实时定量PCR、免疫荧光和组织蛋白电泳方法,分析15例正常前列腺、20例BPH与40例PCa标本中AR的表达情况。结果:实时定量PCR和组织蛋白电泳检测BPH组织与正常前列腺组织中AR的表达量差异无统计学意义(P>0.05)。但免疫荧光检测发现BPH组织中AR蛋白表达量增高。3种方法检测PCa组织中AR表达量较正常前列腺组织和BPH组织增高(P<0.05)。高分化PCa的AR表达比低分化PCa高(P<0.05)。随着临床分期的增高,AR的表达降低(P<0.05),激素非依赖性前列腺癌(HRPC)组织中AR表达最低。结论:AR在PCa组织中的表达较正常前列腺和BPH组织中增高,AR的表达与PCa的分级、分期相关。Objective：To study the expressions of the androgen receptor（AR）in the normal prostate,benigh prostatic hyperplasia and prostate cancer（PCa）,and investigate the relationship of AR with prostatic hyperplasia and PCa.Methods：The expressions of AR were detected in 15 normal prostate,20 benigh prostatic hyperplasia and 40 PCa samples by immunofluorescent staining,real-time PCR and Western blotting.Results：Real-time PCR and Western blotting revealed no statistically significant differences in the expressions of AR between the normal prostate and prostatic hyperplasia groups（P 0.05）,while immunofluorescent staining exhibited an increase of the expression in the BPH tissues.All the three methods showed that the AR expression was significantly higher in the PCa than in the normal prostate and prostatic hyperplasia groups（P 0.05）,in the well differentiated than in the poorly differentiated tumor,and in the early than in the advanced stage（P 0.05）,but the lowest in the hormone-refractory PCa（HRPC）tissue.Conclusion：The expression of AR is higher in PCa than in normal prostate and prostatic hyperplasia tissues,and is correlated with the pathological grade and clinical stage of PCa.

关 键 词：良性前列腺增生 前列腺癌 雄激素受体 

分 类 号：R737.25[医药卫生—肿瘤]